| Autor: |
Uechi K; 1Department of Infectious, Respiratory, and Digestive Medicine, Graduate School of Medicine, University of the Ryukyus, Okinawa, Japan.; 2Division of Clinical Laboratory and Blood Transfusion, University of the Ryukyus Hospital, Okinawa, Japan., Tada T; 3Department of Microbiology, Juntendo University School of Medicine, Tokyo, Japan., Kuwahara-Arai K; 3Department of Microbiology, Juntendo University School of Medicine, Tokyo, Japan., Sekiguchi JI; 4Microbiology Research Division, Kohjin Bio Co., Ltd., Saitama, Japan., Yanagisawa I; 4Microbiology Research Division, Kohjin Bio Co., Ltd., Saitama, Japan., Tome T; 2Division of Clinical Laboratory and Blood Transfusion, University of the Ryukyus Hospital, Okinawa, Japan., Nakasone I; 5Control and Prevention of Infectious Disease, University of the Ryukyus Hospital, Okinawa, Japan., Maeda S; 2Division of Clinical Laboratory and Blood Transfusion, University of the Ryukyus Hospital, Okinawa, Japan., Mya S; 6National Health Laboratory, Yangon, Myanmar., Zan KN; 6National Health Laboratory, Yangon, Myanmar., Tin HH; 6National Health Laboratory, Yangon, Myanmar., Kirikae T; 3Department of Microbiology, Juntendo University School of Medicine, Tokyo, Japan., Fujita J; 1Department of Infectious, Respiratory, and Digestive Medicine, Graduate School of Medicine, University of the Ryukyus, Okinawa, Japan. |
| Abstrakt: |
The modified carbapenem inactivation method (mCIM) is a simple phenotypic screening method for detecting carbapenemase production by Enterobacteriaceae and Pseudomonas aeruginosa. We recently developed another modified carbapenem inactivation method (CIMTris), in which carbapenemase is extracted from bacteria with Tris-HCl buffer, to detect carbapenemase production by Acinetobacter and Pseudomonas species. This study describes an improved carbapenem inactivation method, CIMTrisII, for detecting carbapenemase production by Gram-negative pathogens, including Enterobacteriaceae, Acinetobacter and Pseudomonas species. CIMTrisII was different from CIMTris in the concentration of Meropenem disks (5-µg MEM disks vs. 10-µg MEM disks), the inoculum volume of the bacteria (a 5-µl loopful vs. a 10 µl loopful) and the incubation time (1 vs. 2 h). CIMTrisII showed an overall sensitivity of 99.3 % and an overall specificity of 95.0 % for tested isolates. In comparison, CIMTris showed a sensitivity of 96.1 % and a specificity of 96.3 %, and mCIM showed a sensitivity of 67.1 % and a specificity of 100 %. CIMTrisII is thus deemed useful for detecting carbapenemase production by Gram-negative pathogens. |
