Functionalized core/shell nanofibers for the differentiation of mesenchymal stem cells for vascular tissue engineering.

Autor:	Ezhilarasu H; Department of Mechanical Engineering, Center for Nanofibers & Nanotechnology, Faculty of Engineering, National University of Singapore, Singapore., Sadiq A; Department of Mechanical Engineering, Center for Nanofibers & Nanotechnology, Faculty of Engineering, National University of Singapore, Singapore., Ratheesh G; Institute of Health & Biomedical Innovation, Science & Engineering Faculty, Queensland University of Technology (QUT), Australia., Sridhar S; Department of Mechanical & Construction Engineering, Northumbria University, Newcastle upon Tyne, NE1 8ST, UK., Ramakrishna S; Department of Mechanical Engineering, Center for Nanofibers & Nanotechnology, Faculty of Engineering, National University of Singapore, Singapore., Ab Rahim MH; Faculty of Industrial Sciences & Technology, Universiti Malaysia Pahang, 26300 Gambang, Kuantan, Pahang, Malaysia., Yusoff MM; Faculty of Industrial Sciences & Technology, Universiti Malaysia Pahang, 26300 Gambang, Kuantan, Pahang, Malaysia., Jose R; Faculty of Industrial Sciences & Technology, Universiti Malaysia Pahang, 26300 Gambang, Kuantan, Pahang, Malaysia., Reddy VJ; Department of Mechanical Engineering, Center for Nanofibers & Nanotechnology, Faculty of Engineering, National University of Singapore, Singapore.; Faculty of Industrial Sciences & Technology, Universiti Malaysia Pahang, 26300 Gambang, Kuantan, Pahang, Malaysia.
Jazyk:	angličtina
Zdroj:	Nanomedicine (London, England) [Nanomedicine (Lond)] 2019 Jan; Vol. 14 (2), pp. 201-214. Date of Electronic Publication: 2018 Dec 10.
DOI:	10.2217/nnm-2018-0271
Abstrakt:	Aim: Atherosclerosis is a common cardiovascular disease causing medical problems globally leading to coronary artery bypass surgery. The present study is to fabricate core/shell nanofibers to encapsulate VEGF for the differentiation of mesenchymal stem cells (MSCs) into smooth muscle cells to develop vascular grafts. Materials & Methods: The fabricated core/shell nanofibers contained polycaprolactone/gelatin as the shell, and silk fibroin/VEGF as the core materials. Results: The results observed that the core/shell nanofibers interact to differentiate MSCs into smooth muscle cells by the expression of vascular smooth muscle cell (VSMC) contractile proteins α-actinin, myosin and F-actin. Conclusion: The functionalized polycaprolactone/gelatin/silk fibroin/VEGF (250 ng) core/shell nanofibers were fabricated for the controlled release of VEGF in a persistent manner for the differentiation of MSCs into smooth muscle cells for vascular tissue engineering.
Databáze:	MEDLINE
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