Polysaccharide from a Tunisian red seaweed Chondrus canaliculatus: Structural characteristics, antioxidant activity and in vivo hemato-nephroprotective properties on maneb induced toxicity.

Autor: Jaballi I; Laboratory of Enzyme engineering and Microbiology, National Engineering School in Sfax, University of Sfax, B.P. 1173, 3038 Sfax, Tunisia., Sallem I; Laboratory of Microorganisms and Biomolecules of the Center of Biotechnolgy of Sfax, Sfax, Tunisia., Feki A; Laboratory of Enzyme engineering and Microbiology, National Engineering School in Sfax, University of Sfax, B.P. 1173, 3038 Sfax, Tunisia., Cherif B; Unit of Biotechnology and Pathology, Higher Institute of Biotechnology of Sfax, P.O. Box 261, 3038 Sfax, Tunisia., Kallel C; Laboratory of Hematology, CHU Habib Bourguiba, University of Sfax, 3029 Sfax, Tunisia., Boudawara O; Laboratory of Anatomopathology, CHU Habib Bourguiba, University of Sfax, 3029 Sfax, Tunisia., Jamoussi K; Laboratory of Biochemistry, CHU Hedi Chaker, University of Sfax, 3029 Sfax, Tunisia., Mellouli L; Laboratory of Microorganisms and Biomolecules of the Center of Biotechnolgy of Sfax, Sfax, Tunisia., Nasri M; Laboratory of Enzyme engineering and Microbiology, National Engineering School in Sfax, University of Sfax, B.P. 1173, 3038 Sfax, Tunisia., Amara IB; Laboratory of Enzyme engineering and Microbiology, National Engineering School in Sfax, University of Sfax, B.P. 1173, 3038 Sfax, Tunisia. Electronic address: ibtissembenamara@outlook.fr.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2019 Feb 15; Vol. 123, pp. 1267-1277. Date of Electronic Publication: 2018 Dec 03.
DOI: 10.1016/j.ijbiomac.2018.12.048
Abstrakt: The present study aims to investigate the physicochemical and the structural features of polysaccharide isolated from the red marine macro alga Chondrus canaliculatus (C.C.P) using FT-IR, gel filtration high-pressure chromatograph, HPLC-FID and solid state 13 C NMR analysis. C.C.P was even more tested in vitro for its potential antioxidant properties and in vivo for its hemato-nephroprotective effects against fungicide - maneb (MB) - induced toxicity. Animals treated for 20 days were allocated into six groups per six rats each: group 1 served as vehicle control, group 2 received MB, group 3 received MB + C.C.P (100 mg/kg), group 4 MB + C.C.P (200 mg/kg), group 5 and 6 used as a positive control groups receiving only C.C.P; one 100 and the other 200 mg/kg, respectively. After MB injection, our data displayed a significant disruption in all hematological parameters associated with clear signs of nephrotoxicity. However, co-treatment with C.C.P at two graded doses led to an effective healing process against MB's hematological, biochemical, and histological kidney's oxidative injuries. In summary, our data suggest that C.C.P could be a prospective potent antioxidant, nephro and hemato-protective agent.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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