Targeting myomiRs by tocotrienol-rich fraction to promote myoblast differentiation.

Autor: Razak AM; Department of Biochemistry, Faculty of Medicine, Level 17, Preclinical Building, Universiti Kebangsaan Malaysia Medical Centre (UKMMC), Jalan Yaakob Latif, Bandar Tun Razak, Cheras, 56000 Kuala Lumpur, Malaysia., Khor SC; Department of Biochemistry, Faculty of Medicine, Level 17, Preclinical Building, Universiti Kebangsaan Malaysia Medical Centre (UKMMC), Jalan Yaakob Latif, Bandar Tun Razak, Cheras, 56000 Kuala Lumpur, Malaysia., Jaafar F; Department of Biochemistry, Faculty of Medicine, Level 17, Preclinical Building, Universiti Kebangsaan Malaysia Medical Centre (UKMMC), Jalan Yaakob Latif, Bandar Tun Razak, Cheras, 56000 Kuala Lumpur, Malaysia., Karim NA; Department of Biochemistry, Faculty of Medicine, Level 17, Preclinical Building, Universiti Kebangsaan Malaysia Medical Centre (UKMMC), Jalan Yaakob Latif, Bandar Tun Razak, Cheras, 56000 Kuala Lumpur, Malaysia., Makpol S; Department of Biochemistry, Faculty of Medicine, Level 17, Preclinical Building, Universiti Kebangsaan Malaysia Medical Centre (UKMMC), Jalan Yaakob Latif, Bandar Tun Razak, Cheras, 56000 Kuala Lumpur, Malaysia.
Jazyk: angličtina
Zdroj: Genes & nutrition [Genes Nutr] 2018 Nov 29; Vol. 13, pp. 31. Date of Electronic Publication: 2018 Nov 29 (Print Publication: 2018).
DOI: 10.1186/s12263-018-0618-2
Abstrakt: Background: Several muscle-specific microRNAs (myomiRs) are differentially expressed during cellular senescence. However, the role of dietary compounds on myomiRs remains elusive. This study aimed to elucidate the modulatory role of tocotrienol-rich fraction (TRF) on myomiRs and myogenic genes during differentiation of human myoblasts. Young and senescent human skeletal muscle myoblasts (HSMM) were treated with 50 μg/mL TRF for 24 h before and after inducing differentiation.
Results: The fusion index and myotube surface area were higher ( p  < 0.05) on days 3 and 5 than that on day 1 of differentiation. Ageing reduced the differentiation rate, as observed by a decrease in both fusion index and myotube surface area in senescent cells ( p  < 0.05). Treatment with TRF significantly increased differentiation at days 1, 3 and 5 of young and senescent myoblasts. In senescent myoblasts, TRF increased the expression of miR-206 and miR-486 and decreased PTEN and PAX7 expression . However, the expression of IGF1R was upregulated during early differentiation and decreased at late differentiation when treated with TRF. In young myoblasts, TRF promoted differentiation by modulating the expression of miR-206 , which resulted in the reduction of PAX7 expression and upregulation of IGF1R .
Conclusion: TRF can potentially promote myoblast differentiation by modulating the expression of myomiRs, which regulate the expression of myogenic genes.
Competing Interests: The authors declare that they have no competing interests.Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Databáze: MEDLINE
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