In Vitro Study of Deuterium Effect on Biological Properties of Human Cultured Adipose-Derived Stem Cells.

Autor: Zlatska A; State Institute of Genetic and Regenerative Medicine, National Academy of Medical Sciences of Ukraine, Kyiv, Ukraine.; Biotechnology Laboratory Ilaya Regeneration, Medical Company Ilaya®, Kyiv, Ukraine., Gordiienko I; Biotechnology Laboratory Ilaya Regeneration, Medical Company Ilaya®, Kyiv, Ukraine.; RE Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine., Vasyliev R; State Institute of Genetic and Regenerative Medicine, National Academy of Medical Sciences of Ukraine, Kyiv, Ukraine.; Biotechnology Laboratory Ilaya Regeneration, Medical Company Ilaya®, Kyiv, Ukraine., Zubov D; State Institute of Genetic and Regenerative Medicine, National Academy of Medical Sciences of Ukraine, Kyiv, Ukraine.; Biotechnology Laboratory Ilaya Regeneration, Medical Company Ilaya®, Kyiv, Ukraine., Gubar O; Biotechnology Laboratory Ilaya Regeneration, Medical Company Ilaya®, Kyiv, Ukraine., Rodnichenko A; State Institute of Genetic and Regenerative Medicine, National Academy of Medical Sciences of Ukraine, Kyiv, Ukraine.; Biotechnology Laboratory Ilaya Regeneration, Medical Company Ilaya®, Kyiv, Ukraine., Syroeshkin A; Peoples Friendship University of Russia (RUDN University), 6 Miklukho-Maklaya St., Moscow 117198, Russia., Zlatskiy I; Peoples Friendship University of Russia (RUDN University), 6 Miklukho-Maklaya St., Moscow 117198, Russia.; Dumanskii Institute of Colloid Chemistry and Water Chemistry, National Academy of Sciences of Ukraine, Kyiv, Ukraine.
Jazyk: angličtina
Zdroj: TheScientificWorldJournal [ScientificWorldJournal] 2018 Nov 04; Vol. 2018, pp. 5454367. Date of Electronic Publication: 2018 Nov 04 (Print Publication: 2018).
DOI: 10.1155/2018/5454367
Abstrakt: In current in vitro study we have shown the impact of deuterium content in growth medium on proliferation rate of human cultured adipose-derived stem cells (ADSC). ADSCs have also demonstrated morphological changes when cultured in deuterated growth medium: the cell cultures did not reach confluence but acquired polygonal morphology with pronounced stress fibers. At high deuterium concentrations the ADSCs population doubling time increased which indicated the cell cycle retardation and decrease of cell proliferation rate. The deuterated and deuterium-depleted growth media demonstrated acute and chronic cytotoxicity, respectively. The minimal migration ability was observed in deuterated medium whereas the highest migration activity was observed in the medium with the deuterium content close to natural. The cells in deuterated growth medium demonstrated decrease in metabolic activity after three days in culture. In contrast, in deuterium-depleted medium there was an increase in ADSC metabolic activity.
Databáze: MEDLINE
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