First reported nosocomial outbreak of Serratia marcescens harboring bla IMP-4 and bla VIM-2 in a neonatal intensive care unit in Cairo, Egypt.

Autor: Ghaith DM; Department of Clinical and Chemical Pathology, Faculty of Medicine, Cairo University, Cairo, Egypt., Zafer MM; Department of Microbiology and Immunology, Faculty of Pharmacy, Ahram Canadian University, Giza, Egypt, mai_zafer@hotmail.com., Ismail DK; Department of Clinical and Chemical Pathology, Faculty of Medicine, Cairo University, Cairo, Egypt., Al-Agamy MH; Department of Pharmaceutics, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.; Department of Microbiology and Immunology, Faculty of Pharmacy, Al-Azhar University, Cairo, Egypt., Bohol MFF; Department of Infection and Immunity, Research Center, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia., Al-Qahtani A; Department of Infection and Immunity, Research Center, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia., Al-Ahdal MN; Department of Infection and Immunity, Research Center, King Faisal Specialist Hospital and Research Center, Riyadh, Saudi Arabia., Elnagdy SM; Department of Botany and Microbiology, Faculty of Science, Cairo University, Cairo, Egypt., Mostafa IY; Department of Microbiology, Faculty of Dentistry and Oral Medicine, Future University, Cairo, Egypt.
Jazyk: angličtina
Zdroj: Infection and drug resistance [Infect Drug Resist] 2018 Nov 08; Vol. 11, pp. 2211-2217. Date of Electronic Publication: 2018 Nov 08 (Print Publication: 2018).
DOI: 10.2147/IDR.S174869
Abstrakt: Introduction: Serratia marcescens is a significant hospital-acquired pathogen, and many outbreaks of S. marcescens infection have been reported in neonates. We report a sudden breakout of S. marcescens harboring the bla IMP-4 and bla VIM-2 metallo-β-lactamase (MBL) genes that occurred from March to August 2015 in the neonatal intensive care unit of Cairo University Hospital, Cairo, Egypt.
Methods: During the study period, 40 nonduplicate clinical isolates of S. marcescens were collected from blood culture samples. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry was used to identify each isolate. Then, minimum inhibitory concentrations of different antibiotics were assessed by the Vitek 2 compact system. Screening of the MBL genes bla IMP , bla VIM , bla SIM-1 , bla SPM-1 , and bla GIM-1 as well as the carbapenemase genes KPC, NDM, OXA-48, SME-1, and SME-2 were evaluated. Pulsed field gel electrophoresis was preformed to detect the genetic relationship of the isolates.
Results: Analysis showed that 37.5% of the S. marcescens clinical isolates were resistant to meropenem (minimum inhibitory concentrations ≥ 2 µg/mL), and bla IMP-4 and bla VIM-2 were the most prevalent MBL genes (42.5% and 37.5%, respectively). None of the other investigated genes were observed. Pulsed field gel electrophoresis typing revealed two discrete clones; 33/40 (82.5%) were pulsotype A and 7/40 (17.5%) were pulsotype B.
Conclusion: Here, we report for the first time the detection of MBL-producing S. marcescens isolates, particularly IMP-4 and VIM-2 recovered from inpatients with bacteremias from the intensive care unit at Cairo University Hospital.
Competing Interests: Disclosure The authors report no conflicts of interest in this work.
Databáze: MEDLINE
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