| Autor: |
Reyes-Hernández CG; Department of Physiology, Faculty of Medicine, University Autónoma of Madrid, 28029 Madrid, Spain. cynthia.grh3@yahoo.com., Ramiro-Cortijo D; Department of Physiology, Faculty of Medicine, University Autónoma of Madrid, 28029 Madrid, Spain. david.ramiro@uam.es., Rodríguez-Rodríguez P; Department of Physiology, Faculty of Medicine, University Autónoma of Madrid, 28029 Madrid, Spain. pilar.rodriguezr@uam.es., Giambelluca S; Fondazione Istituto di Ricerca Pediatrica Cittá della Speranza, 35129 Padova, Italy. sonia.giambelluca@gmail.com., Simonato M; Fondazione Istituto di Ricerca Pediatrica Cittá della Speranza, 35129 Padova, Italy. lab2nut@gmail.com., González MDC; Department of Physiology, Faculty of Medicine, University Autónoma of Madrid, 28029 Madrid, Spain. m.c.gonzalez@uam.es., López de Pablo AL; Department of Physiology, Faculty of Medicine, University Autónoma of Madrid, 28029 Madrid, Spain. angel.lopezdepablo@uam.es., López-Giménez MDR; Department of Epidemiology, Public Health & Microbiology, Faculty of Medicine, University Autónoma of Madrid, 28029 Madrid, Spain. mrosario.lopez@uam.es., Cogo P; Division of Pediatrics, Department of Medicine, University of Udine, 33100 Udine, Italy. paola.cogo@uniud.it., Sáenz de Pipaón M; Department of Neonatology, La Paz Hospital-University Autónoma of Madrid, 28046 Madrid, Spain. miguel.saenz@salud.madrid.org.; Carlos III Health Institute, Maternal and Child Health and Development Research Network, 28029 Madrid, Spain. miguel.saenz@salud.madrid.org., Carnielli VP; Division of Neonatology, Department of Clinical Sciences, Polytechnic University of Marche, 60121 Ancona, Italy. v.carnielli@staff.univpm.it.; Azienda Ospedaliero, Universitaria Ospedali Riuniti, 60030 Ancona, Italy. v.carnielli@staff.univpm.it., Arribas SM; Department of Physiology, Faculty of Medicine, University Autónoma of Madrid, 28029 Madrid, Spain. silvia.arribas@uam.es. |
| Abstrakt: |
Arachidonic and docosahexaenoic acids (ARA and DHA) are important during pregnancy. However, the effects of dietary supplementation on fetal growth and oxidative stress are inconclusive. We aimed to assess the effect of high ARA and DHA diet during rat gestation on: (1) ARA and DHA availability in plasma and placenta, (2) fetal growth, and (3) placental oxidative stress, analyzing the influence of sex. Experimental diet (ED) was prepared by substituting soybean oil in the control diet (CD) by a fungi/algae-based oil containing ARA and DHA (2:1). Rats were fed with CD or ED during gestation; plasma, placenta, and fetuses were obtained at gestational day 20. DHA, ARA, and their precursors were analyzed in maternal plasma and placenta by gas chromatography/mass spectrophotometry. Fetuses and placentas were weighed, the proportion of fetuses with intrauterine growth restriction (IUGR) determined, and placental lipid and protein oxidation analyzed. ED fetuses exhibited lower body weight compared to CD, being >40% IUGR; fetal weight negatively correlated with maternal plasma ARA, but not DHA. Only ED female placenta exhibited higher lipid and protein oxidation compared to its CD counterparts; lipid peroxidation is negatively associated with fetal weight. In conclusion, high ARA during gestation associates with IUGR, through placental oxidative stress, with females being more susceptible. |
