Changes in hemocytes of Biomphalaria glabrata infected with Echinostoma paraensei and exposed to glyphosate-based herbicide.

Autor: Monte TCC; Biodiversity and Health, Oswaldo Cruz Institute, Fiocruz, Avenida Brasil 4365, Manguinhos, Zip code: 21040-360, Rio de Janeiro, Brazil; Laboratory of Biology and Parasitology of Wild Mammal Reservoirs, Oswaldo Cruz Institute, Fiocruz, Avenida Brasil 4365, Manguinhos, Zip code: 21040-360, Rio de Janeiro, Brazil., Chometon TQ; Laboratory of Immunoparasitology, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro, Brazil; Flow Cytometry Cell Sorting Core Facility, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro, Brazil., Bertho AL; Laboratory of Immunoparasitology, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro, Brazil; Flow Cytometry Cell Sorting Core Facility, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro, Brazil. Electronic address: alvaro.bertho@ioc.fiocruz.br., de Moura VS; Laboratory of Biology and Parasitology of Wild Mammal Reservoirs, Oswaldo Cruz Institute, Fiocruz, Avenida Brasil 4365, Manguinhos, Zip code: 21040-360, Rio de Janeiro, Brazil., de Vasconcellos MC; Laboratory of Environmental Health Assessment and Promotion, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro, Brazil., Garcia J; Laboratory of Biology and Parasitology of Wild Mammal Reservoirs, Oswaldo Cruz Institute, Fiocruz, Avenida Brasil 4365, Manguinhos, Zip code: 21040-360, Rio de Janeiro, Brazil., Ferraz-Nogueira R; Laboratory of Immunoparasitology, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro, Brazil; Flow Cytometry Cell Sorting Core Facility, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro, Brazil., Maldonado Júnior A; Laboratory of Biology and Parasitology of Wild Mammal Reservoirs, Oswaldo Cruz Institute, Fiocruz, Avenida Brasil 4365, Manguinhos, Zip code: 21040-360, Rio de Janeiro, Brazil., Faro MJ; Laboratory of Biology and Parasitology of Wild Mammal Reservoirs, Oswaldo Cruz Institute, Fiocruz, Avenida Brasil 4365, Manguinhos, Zip code: 21040-360, Rio de Janeiro, Brazil.
Jazyk: angličtina
Zdroj: Journal of invertebrate pathology [J Invertebr Pathol] 2019 Jan; Vol. 160, pp. 67-75. Date of Electronic Publication: 2018 Dec 01.
DOI: 10.1016/j.jip.2018.11.007
Abstrakt: The immune system of snails is highly sensitive to pollutants, which can suppress its immune response. We investigated the effects of exposure to the glyphosate-based herbicide Roundup® Original on the snail Biomphalaria glabrata infected by the platyhelminth Echinostoma paraensei by evaluating changes in the snail's internal defense system. Four cohorts were studied: control group, infected snails, snails treated with Roundup®, and snails infected and treated with Roundup®. The hemocyte viability was assessed, morphological differentiation of cells was observed and flow cytometry was performed to determine the morphology, viability and the lectin expression profiles. The frequencies of dead hemocytes were lower in the infected group and higher in both pesticide treated groups. Three cell types were identified: blast-like cells, hyalinocytes and granulocytes. The highest number of all types of hemocytes, as well as the highest number of dead cells, were observed in the infected, pesticide-treated group. The association between infection and herbicide exposure greatly increased the frequency of dead hemocytes, suggesting that this condition impairs the internal defense system of B. glabrata making the snails more vulnerable to parasitic infections.
(Copyright © 2018 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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