| Autor: |
Lund J; Department of Pharmaceutical Biosciences, School of Pharmacy, University of Oslo, Oslo, Norway. jenny.lund@farmasi.uio.no., Helle SA; Department of Pharmaceutical Biosciences, School of Pharmacy, University of Oslo, Oslo, Norway., Li Y; Department of Nutrition, Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway., Løvsletten NG; Department of Pharmaceutical Biosciences, School of Pharmacy, University of Oslo, Oslo, Norway., Stadheim HK; Department of Physical Performance, Norwegian School of Sport Sciences, Oslo, Norway., Jensen J; Department of Physical Performance, Norwegian School of Sport Sciences, Oslo, Norway., Kase ET; Department of Pharmaceutical Biosciences, School of Pharmacy, University of Oslo, Oslo, Norway., Thoresen GH; Department of Pharmaceutical Biosciences, School of Pharmacy, University of Oslo, Oslo, Norway.; Department of Pharmacology, Institute of Clinical Medicine, University of Oslo, Oslo, Norway., Rustan AC; Department of Pharmaceutical Biosciences, School of Pharmacy, University of Oslo, Oslo, Norway. |
| Abstrakt: |
In this study we compared fatty acid (FA) metabolism in myotubes established from athletic and sedentary young subjects. Six healthy sedentary (maximal oxygen uptake (VO 2max ) ≤ 46 ml/kg/min) and six healthy athletic (VO 2max > 60 ml/kg/min) young men were included. Myoblasts were cultured and differentiated to myotubes from satellite cells isolated from biopsy of musculus vastus lateralis. FA metabolism was studied in myotubes using [ 14 C]oleic acid. Lipid distribution was assessed by thin layer chromatography, and FA accumulation, lipolysis and re-esterification were measured by scintillation proximity assay. Gene and protein expressions were studied. Myotubes from athletic subjects showed lower FA accumulation, lower incorporation of FA into total lipids, triacylglycerol (TAG), diacylglycerol and cholesteryl ester, higher TAG-related lipolysis and re-esterification, and higher complete oxidation and incomplete β-oxidation of FA compared to myotubes from sedentary subjects. mRNA expression of the mitochondrial electron transport chain complex III gene UQCRB was higher in cells from athletic compared to sedentary. Myotubes established from athletic subjects have higher lipid turnover and oxidation compared to myotubes from sedentary subjects. Our findings suggest that cultured myotubes retain some of the phenotypic traits of their donors. |
