Expression of CK7 and CDKN2 in Cervical Intraepithelial Neoplasia and Correlation with Clinical Outcome.

Autor:	DE Lima TM; Gynecological Disease Prevention Nucleus (NUPREV), Department of Gynecology, Federal University of São Paulo, Sao Paulo, Brazil., DE Azevedo Focchi GR; Department of Pathology, Federal University of São Paulo, Sao Paulo, Brazil., DE Almeida BC; Structural and Molecular Gynecology Laboratory (LIM 58), Department of Obstetrics and Gynecology, Clinical Hospital of University of São Paulo Medical School, Sao Paulo, Brazil., Belfort-Mattos PN; Gynecological Disease Prevention Nucleus (NUPREV), Department of Gynecology, Federal University of São Paulo, Sao Paulo, Brazil., Heinke T; Department of Pathology, Federal University of São Paulo, Sao Paulo, Brazil thaish1978@gmail.com., DE Góis Speck NM; Gynecological Disease Prevention Nucleus (NUPREV), Department of Gynecology, Federal University of São Paulo, Sao Paulo, Brazil.
Jazyk:	angličtina
Zdroj:	Anticancer research [Anticancer Res] 2018 Dec; Vol. 38 (12), pp. 6673-6681.
DOI:	10.21873/anticanres.13035
Abstrakt:	Aim: To evaluate cyclin-dependent kinase inhibitor 2A (CDKN2A) and cytokeratin 7 (CK7) expression in cervical intraepithelial neoplasia (CIN) formalin-fixed samples. Materials and Methods: Staining with antibody clones G175-405 for CDKN2A and OV-TL 12/30 for CK7 were evaluated and the detection of protein expressions were compared in 147 patients with CIN. Results: Clinical follow-up of patients with CIN1 and CIN2 showed that most patients had a favorable outcome. Single CDKN2A or CK7 expression and their combined expression had a greater sensitivity and negative predictive value in CIN1, corresponding to the non-development of the disease. The positive predictive value of CDKN2A was greater than that of CK7. Combined expression of CDKN2A and CK7 showed that the sensitivity, specificity, positive predictive values, and negative predictive values had their maximum index in the CIN1 group. Analysis of combined expression of CDKN2A and CK7 showed that 85.7% of patients presented unfavorable clinical outcomes, with positive expression for both markers identified in CIN2. Conclusion: Combined expression of CK7 and CDKN2A was associated with a better diagnosis of CIN, and negative expression in CIN1/2 groups had a greater negative predictive value for patient clinical outcome. (Copyright© 2018, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu