| Autor: |
Skalickova S; Central Laboratory, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho 1941/1, 612 42 Brno, Czech Republic., Docekalova M; Central Laboratory, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho 1941/1, 612 42 Brno, Czech Republic., Stankova M; Central Laboratory, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho 1941/1, 612 42 Brno, Czech Republic., Uhlirova D; Central Laboratory, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho 1941/1, 612 42 Brno, Czech Republic., Ruttkay-Nedecky B; Central Laboratory, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho 1941/1, 612 42 Brno, Czech Republic., Fernandez C; School of Pharmacy and Life Sciences, Robert Gordon University, Aberdeen AB107GJ, United Kingdom., Kepinska M; Wroclaw Medical University, Faculty of Pharmacy with Division of Laboratory Diagnostics, Department of Biomedical and Environmental Analyses, Borowska 211, 50-556 Wroclaw, Poland., Milnerowicz H; Wroclaw Medical University, Faculty of Pharmacy with Division of Laboratory Diagnostics, Department of Biomedical and Environmental Analyses, Borowska 211, 50-556 Wroclaw, Poland., Kizek R; Central Laboratory, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, Palackeho 1941/1, 612 42 Brno, Czech Republic. |
| Abstrakt: |
Modern anticancer therapy aims to increase the effectiveness of tumor treatment. The aim of this work was to propose a new nanotransporter for targeted delivery of anthracycline antibiotics, which is characterized by its bioavailability, increased uptake of the drug from the bloodstream at the site of the tumor tissue as well as low toxicity to non-target tissue. Chitosan nanoparticles have attracted great attention in the field of drug delivery due to their stability, low toxicity and easy preparation. Deacetylated chitosan skeleton is composed of glucosamine units and has a high density of charged amino groups which allow strong electrostatic interactions with biomolecules, transition metals (Zn, Se) and peptides. We obtained an effective level of chitosan encapsulation, 20%. Electrochemical detection of the bounded Zn 2+ ions into the chitosan structure showed a potential shift from -0.99 to -0.93 V. This result proved the formation of a chitosan-zinc complex. The ability of metallothione to quench the 2,2-diphenyl-1-picrylhydrazyl radical in the presence of 50 μ M doxorubicin was confirmed by the change of relative absorbance over the range from 50 to 60%. |
