Effect of C-terminal His-tag and purification routine on the activity and structure of the metalloenzyme, l-alanyl-d-glutamate peptidase of the bacteriophage T5.

Autor: Kutyshenko VP; Institute of Theoretical and Experimental Biophysics RAS, Pushchino, Moscow Region 142290, Russia. Electronic address: kutyshenko@rambler.ru., Mikoulinskaia GV; Branch of Shemyakin & Ovchinnikov's Institute of Bioorganic Chemistry RAS, Pushchino, Moscow Region 142290, Russia. Electronic address: mikulinskaya@bibch.ru., Chernyshov SV; Branch of Shemyakin & Ovchinnikov's Institute of Bioorganic Chemistry RAS, Pushchino, Moscow Region 142290, Russia., Yegorov AY; Institute of Theoretical and Experimental Biophysics RAS, Pushchino, Moscow Region 142290, Russia. Electronic address: a.egorov@vega.protres.ru., Prokhorov DA; Institute of Theoretical and Experimental Biophysics RAS, Pushchino, Moscow Region 142290, Russia., Uversky VN; Department of Molecular Medicine and USF Health Byrd Alzheimer's Research Institute, Morsani College of Medicine, University of South Florida, Tampa, FL, USA; Institute for Biological Instrumentation, Russian Academy of Sciences, Pushchino, Moscow Region 142290, Russia. Electronic address: vuversky@health.usf.edu.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2019 Mar 01; Vol. 124, pp. 810-818. Date of Electronic Publication: 2018 Nov 28.
DOI: 10.1016/j.ijbiomac.2018.11.219
Abstrakt: In this work, we studied the effect of the C-terminally attached poly-histidine tag (His-tag), as well as the peculiarities of the protein purification procedure by the immobilized metal affinity chromatography (IMAC) on the activity and structure of the metalloenzyme, l-alanyl-d-glutamate peptidase of bacteriophage T5 (EndoT5), whose zinc binding site and catalytic aspartate are located near the C-terminus. By itself, His-tag did not have a significant effect on either activity or folding of the polypeptide chain, nor on the binding of zinc and calcium ions to the protein. However, the His-tagged EndoT5 samples had low shelf-life, with storage of these samples resulting in an increased propensity for protein self-association and decreased enzymatic activity of EndoT5. Furthermore, disastrous effects on the activity of the enzyme were exerted by the presence of imidazole and nickel ions accompanying metal chelate chromatography. The activity of the protein can be restored by thorough washing off of these low molecular impurities via the prolonged dialysis of the His-tagged EndoT5 samples at the specifically elaborated conditions.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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