Efficient synthesis and characterisation of the amyloid beta peptide, Aβ 1-42 , using a double linker system.

Autor:	Kasim JK; School of Biological Sciences, The University of Auckland, 3A Symonds Street, Auckland 1010, New Zealand. paul.harris@auckland.ac.nz., Kavianinia I; School of Biological Sciences, The University of Auckland, 3A Symonds Street, Auckland 1010, New Zealand. paul.harris@auckland.ac.nz and Maurice Wilkins Centre for Molecular Biodiscovery, The University of Auckland, 3 Symonds St, Auckland 1010, New Zealand and School of Chemical Sciences, The University of Auckland, 23 Symonds Street, Auckland 1010, New Zealand. m.brimble@auckland.ac.nz., Ng J; School of Biological Sciences, The University of Auckland, 3A Symonds Street, Auckland 1010, New Zealand. paul.harris@auckland.ac.nz and Brain Research New Zealand Rangahau Roro Aotearoa and Centre for Brain Research, Auckland 1010, New Zealand., Harris PWR; School of Biological Sciences, The University of Auckland, 3A Symonds Street, Auckland 1010, New Zealand. paul.harris@auckland.ac.nz and Maurice Wilkins Centre for Molecular Biodiscovery, The University of Auckland, 3 Symonds St, Auckland 1010, New Zealand and School of Chemical Sciences, The University of Auckland, 23 Symonds Street, Auckland 1010, New Zealand. m.brimble@auckland.ac.nz., Birch NP; School of Biological Sciences, The University of Auckland, 3A Symonds Street, Auckland 1010, New Zealand. paul.harris@auckland.ac.nz and Brain Research New Zealand Rangahau Roro Aotearoa and Centre for Brain Research, Auckland 1010, New Zealand., Brimble MA; School of Biological Sciences, The University of Auckland, 3A Symonds Street, Auckland 1010, New Zealand. paul.harris@auckland.ac.nz and Maurice Wilkins Centre for Molecular Biodiscovery, The University of Auckland, 3 Symonds St, Auckland 1010, New Zealand and School of Chemical Sciences, The University of Auckland, 23 Symonds Street, Auckland 1010, New Zealand. m.brimble@auckland.ac.nz.
Jazyk:	angličtina
Zdroj:	Organic & biomolecular chemistry [Org Biomol Chem] 2018 Dec 19; Vol. 17 (1), pp. 30-34.
DOI:	10.1039/c8ob02929f
Abstrakt:	The amyloidogenic Aβ42 peptide was efficiently prepared using a double linker system, markedly improving solubility and chromatographic peak resolution, thus enabling full characterisation using standard techniques. The tag was readily cleaved with sodium hydroxide and removed by aqueous extraction, affording Aβ42 in high purity and yield for biophysical characterisation studies.
Databáze:	MEDLINE
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