Voxel-Based Statistical Analysis of 3D Immunostained Tissue Imaging.

Autor:	Vandenberghe ME; CEA, DRF, Institut François Jacob Molecular Imaging Research Center, Fontenay-aux-Roses, France.; Neurodegenerative Diseases Laboratory, CNRS, CEA, Paris-Sud University, Paris-Saclay University UMR9199, Fontenay-aux-Roses, France., Souedet N; CEA, DRF, Institut François Jacob Molecular Imaging Research Center, Fontenay-aux-Roses, France.; Neurodegenerative Diseases Laboratory, CNRS, CEA, Paris-Sud University, Paris-Saclay University UMR9199, Fontenay-aux-Roses, France., Hérard AS; CEA, DRF, Institut François Jacob Molecular Imaging Research Center, Fontenay-aux-Roses, France.; Neurodegenerative Diseases Laboratory, CNRS, CEA, Paris-Sud University, Paris-Saclay University UMR9199, Fontenay-aux-Roses, France., Ayral AM; INSERM U1167, Institut Pasteur de Lille Université Lille-Nord de France, Lille, France., Letronne F; INSERM U1167, Institut Pasteur de Lille Université Lille-Nord de France, Lille, France., Balbastre Y; CEA, DRF, Institut François Jacob Molecular Imaging Research Center, Fontenay-aux-Roses, France.; Neurodegenerative Diseases Laboratory, CNRS, CEA, Paris-Sud University, Paris-Saclay University UMR9199, Fontenay-aux-Roses, France., Sadouni E; CEA, DRF, Institut François Jacob Molecular Imaging Research Center, Fontenay-aux-Roses, France.; Neurodegenerative Diseases Laboratory, CNRS, CEA, Paris-Sud University, Paris-Saclay University UMR9199, Fontenay-aux-Roses, France., Hantraye P; CEA, DRF, Institut François Jacob Molecular Imaging Research Center, Fontenay-aux-Roses, France.; Neurodegenerative Diseases Laboratory, CNRS, CEA, Paris-Sud University, Paris-Saclay University UMR9199, Fontenay-aux-Roses, France., Dhenain M; CEA, DRF, Institut François Jacob Molecular Imaging Research Center, Fontenay-aux-Roses, France.; Neurodegenerative Diseases Laboratory, CNRS, CEA, Paris-Sud University, Paris-Saclay University UMR9199, Fontenay-aux-Roses, France., Frouin F; Laboratoire Imagerie Moléculaire in vivo (IMIV UMR 1023 Inserm/CEA/Université Paris Sud - ERL 9218 CNRS) Orsay, France., Lambert JC; INSERM U1167, Institut Pasteur de Lille Université Lille-Nord de France, Lille, France., Delzescaux T; CEA, DRF, Institut François Jacob Molecular Imaging Research Center, Fontenay-aux-Roses, France.; Neurodegenerative Diseases Laboratory, CNRS, CEA, Paris-Sud University, Paris-Saclay University UMR9199, Fontenay-aux-Roses, France.
Jazyk:	angličtina
Zdroj:	Frontiers in neuroscience [Front Neurosci] 2018 Nov 15; Vol. 12, pp. 754. Date of Electronic Publication: 2018 Nov 15 (Print Publication: 2018).
DOI:	10.3389/fnins.2018.00754
Abstrakt:	Recently developed techniques to visualize immunostained tissues in 3D and in large samples have expanded the scope of microscopic investigations at the level of the whole brain. Here, we propose to adapt voxel-based statistical analysis to 3D high-resolution images of the immunostained rodent brain. The proposed approach was first validated with a simulation dataset with known cluster locations. Then, it was applied to characterize the effect of ADAM30, a gene involved in the metabolism of the amyloid precursor protein, in a mouse model of Alzheimer's disease. This work introduces voxel-based analysis of 3D immunostained microscopic brain images and, therefore, opens the door to localized whole-brain exploratory investigation of pathological markers and cellular alterations.
Databáze:	MEDLINE
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