CRISPR and Target-Specific DNA Endonucleases for Efficient DNA Knock-in in Eukaryotic Genomes.
| Autor: | Lee SH; National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju 28116, Korea., Kim S; Department of Pathology, College of Medicine, Kyung Hee University, Seoul 02447, Korea., Hur JK; Department of Pathology, College of Medicine, Kyung Hee University, Seoul 02447, Korea. |
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| Jazyk: | angličtina |
| Zdroj: | Molecules and cells [Mol Cells] 2018 Nov 30; Vol. 41 (11), pp. 943-952. Date of Electronic Publication: 2018 Nov 14. |
| DOI: | 10.14348/molcells.2018.0408 |
| Abstrakt: | The discovery and mechanistic understanding of target-specific genome engineering technologies has led to extremely effective and specific genome editing in higher organisms. Target-specific genetic modification technology is expected to have a leading position in future gene therapy development, and has a ripple effect on various basic and applied studies. However, several problems remain and hinder efficient and specific editing of target genomic loci. The issues are particularly critical in precise targeted insertion of external DNA sequences into genomes. Here, we discuss some recent efforts to overcome such problems and present a perspective of future genome editing technologies. |
| Databáze: | MEDLINE |
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