Der p 1-specific regulatory T-cell response during house dust mite allergen immunotherapy.

Autor: Boonpiyathad T; Swiss Institute of Allergy and Asthma Research, University of Zurich, Davos, Switzerland.; Christine Kühne-Center for Allergy Research and Education, Davos, Switzerland.; Department of Medicine, Phramongkutklao Hospital, Bangkok, Thailand.; Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand., Sokolowska M; Swiss Institute of Allergy and Asthma Research, University of Zurich, Davos, Switzerland.; Christine Kühne-Center for Allergy Research and Education, Davos, Switzerland., Morita H; Swiss Institute of Allergy and Asthma Research, University of Zurich, Davos, Switzerland.; Department of Allergy and Clinical Immunology, National Research Institute for Child Health and Development, Tokyo, Japan., Rückert B; Swiss Institute of Allergy and Asthma Research, University of Zurich, Davos, Switzerland., Kast JI; Swiss Institute of Allergy and Asthma Research, University of Zurich, Davos, Switzerland., Wawrzyniak M; Swiss Institute of Allergy and Asthma Research, University of Zurich, Davos, Switzerland., Sangasapaviliya A; Department of Medicine, Phramongkutklao Hospital, Bangkok, Thailand., Pradubpongsa P; Department of Medicine, Phramongkutklao Hospital, Bangkok, Thailand., Fuengthong R; Department of Medicine, Phramongkutklao Hospital, Bangkok, Thailand., Thantiworasit P; Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand., Sirivichayakul S; Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand., Kwok WW; Benaroya Research Institute at Virginia Mason, Seattle, Washington.; Department of Immunology, University of Washington, Seattle, Washington., Ruxrungtham K; Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand., Akdis M; Swiss Institute of Allergy and Asthma Research, University of Zurich, Davos, Switzerland., Akdis CA; Swiss Institute of Allergy and Asthma Research, University of Zurich, Davos, Switzerland.; Christine Kühne-Center for Allergy Research and Education, Davos, Switzerland.
Jazyk: angličtina
Zdroj: Allergy [Allergy] 2019 May; Vol. 74 (5), pp. 976-985. Date of Electronic Publication: 2019 Jan 13.
DOI: 10.1111/all.13684
Abstrakt: Background: Allergen-specific immunotherapy (AIT) is the only available treatment for allergic diseases that can induce specific immune tolerance to allergens. The key mechanisms involved in this process include changes in allergen-specific regulatory T (Treg) cells.
Methods: We studied 25 allergic rhinitis patients undergoing subcutaneous house dust mite-specific immunotherapy. Peripheral blood mononuclear cells were studied before and after 10, 30 weeks, and 3 years of AIT. Der p 1-specific T regulatory cell responses were investigated by characterization of Der p 1-MHC class II tetramer-positive cells and correlated with nasal symptom score.
Results: Twelve of 25 AIT patients matched with their MHC class II expression to the Der p 1 peptide-MHC class II tetramers. A significant increase in the numbers of Der p 1-specific FOXP3 + Helios + CD25 + CD127 - Treg cells after 30 weeks was observed, which slightly decreased after 3 years of AIT. In contrast, Der p 1-specific immunoglobulin-like transcript 3 (ILT3) + CD25 + Treg cells decreased substantially from baseline after 3 years of AIT. ILT3 + Treg cells displayed compromised suppressive function and low FOXP3 expression. In addition, Der p 1-specific IL-10 and IL-22 responses have increased after 30 weeks, but only IL-10 + Der p 1-specific Treg cells remained present at high frequency after 3 years of AIT. Increased number of FOXP3 + Helios + and IL-10 + and decreased ILT3 + Treg cell responses correlated with improved allergic symptoms.
Conclusion: The results indicate that AIT involves upregulation of the activated allergen-specific Treg cells and downregulation of dysfunctional allergen-specific Treg cell subset. Correction of dysregulated Treg cells responses during AIT is associated with improved clinical response.
(© 2018 EAACI and John Wiley and Sons A/S. Published by John Wiley and Sons Ltd.)
Databáze: MEDLINE
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