Imaging Insulin Secretion from Mouse Pancreas by MRI Is Improved by Use of a Zinc-Responsive MRI Sensor with Lower Affinity for Zn 2+ Ions.

Autor: Martins AF; Department of Chemistry and Biochemistry , University of Texas at Dallas , Richardson , Texas 75080 , United States.; Advanced Imaging Research Center , University of Texas Southwestern Medical Center , Dallas , Texas 75390-8568 , United States., Clavijo Jordan V; Advanced Imaging Research Center , University of Texas Southwestern Medical Center , Dallas , Texas 75390-8568 , United States., Bochner F; Department of Biological Regulation , The Weizmann Institute of Science , 7610001 Rehovot , Israel., Chirayil S; Advanced Imaging Research Center , University of Texas Southwestern Medical Center , Dallas , Texas 75390-8568 , United States., Paranawithana N; Department of Chemistry and Biochemistry , University of Texas at Dallas , Richardson , Texas 75080 , United States., Zhang S; Advanced Imaging Research Center , University of Texas Southwestern Medical Center , Dallas , Texas 75390-8568 , United States., Lo ST; Advanced Imaging Research Center , University of Texas Southwestern Medical Center , Dallas , Texas 75390-8568 , United States., Wen X; Advanced Imaging Research Center , University of Texas Southwestern Medical Center , Dallas , Texas 75390-8568 , United States., Zhao P; Department of Chemistry and Biochemistry , University of Texas at Dallas , Richardson , Texas 75080 , United States., Neeman M; Department of Biological Regulation , The Weizmann Institute of Science , 7610001 Rehovot , Israel., Sherry AD; Department of Chemistry and Biochemistry , University of Texas at Dallas , Richardson , Texas 75080 , United States.; Advanced Imaging Research Center , University of Texas Southwestern Medical Center , Dallas , Texas 75390-8568 , United States.
Jazyk: angličtina
Zdroj: Journal of the American Chemical Society [J Am Chem Soc] 2018 Dec 19; Vol. 140 (50), pp. 17456-17464. Date of Electronic Publication: 2018 Dec 11.
DOI: 10.1021/jacs.8b07607
Abstrakt: It has been demonstrated that divalent zinc ions packaged with insulin in β-cell granules can be detected by MRI during glucose-stimulated insulin secretion using a gadolinium-based Zn 2+ -sensitive agent. This study was designed to evaluate whether a simpler agent design having single Zn 2+ -sensing moieties but with variable Zn 2+ binding affinities might also detect insulin secretion from the pancreas. Using an implanted MR-compatible window designed to hold the pancreas in a fixed position for imaging, we now demonstrate that focally intense "hot spots" can be detected in the tail of the pancreas using these agents after administration of glucose to stimulate insulin secretion. Histological staining of the same tissue verified that the hot spots identified by imaging correspond to clusters of islets, perhaps reflecting first-responder islets that are most responsive to a sudden increase in glucose. A comparison of images obtained when using a high-affinity Zn 2+ sensor versus a lower-affinity sensor showed that the lower-affinity sensors produced the best image contrast. An equilibrium model that considers all possible complexes formed between Zn 2+ , the GdL sensor, and HSA predicts that a GdL sensor with lower affinity for Zn 2+ generates a lower background signal from endogenous Zn 2+ prior to glucose-stimulated insulin secretion (GSIS) and that the weaker binding affinity agent is more responsive to a further increase in Zn 2+ concentration near β-cells after GSIS. These model predictions are consistent with the in vivo imaging observations.
Databáze: MEDLINE
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