Biomimetic proteoglycans can molecularly engineer early osteoarthritic cartilage in vivo.

Autor: Phillips ER; Department of Materials Science and Engineering, College of Engineering, Drexel University, 3141 Chestnut St, Philadelphia, Pennsylvania, 19104., Prudnikova K; Department of Materials Science and Engineering, College of Engineering, Drexel University, 3141 Chestnut St, Philadelphia, Pennsylvania, 19104., Bui T; College of Medicine, Drexel University, 2900 W Queen Lane, Philadelphia, Pennsylvania, 19129., Taylor AJ; College of Medicine, Drexel University, 2900 W Queen Lane, Philadelphia, Pennsylvania, 19129., Galindo DA; Philadelphia College of Osteopathic Medicine, 4170 City Line Avenue, Philadelphia, Pennsylvania, 19131., Huneke RB; Department of Microbiology and Immunology, College of Medicine, Drexel University, 245 N 15th Street, Philadelphia, Pennsylvania, 19102., Hou JS; Department of Pathology and Laboratory Medicine, College of Medicine, Drexel University, 245 North 15th Street, Philadelphia, Pennsylvania, 19102., Mulcahey MK; Department of Orthopaedic Surgery, Tulane University School of Medicine, 1430 Tulane Avenue Box 8632 Box 8632, New Orleans, Louisiana, 70112., Marcolongo MS; Department of Materials Science and Engineering, College of Engineering, Drexel University, 3141 Chestnut St, Philadelphia, Pennsylvania, 19104.
Jazyk: angličtina
Zdroj: Journal of orthopaedic research : official publication of the Orthopaedic Research Society [J Orthop Res] 2019 Feb; Vol. 37 (2), pp. 403-411. Date of Electronic Publication: 2019 Jan 03.
DOI: 10.1002/jor.24193
Abstrakt: Biomimetic proteoglycans (BPGs) have the potential to treat osteoarthritis (OA) given that these molecules mimic the structure and properties of natural proteoglycans, which are significantly reduced in OA. We examined the effects of BPGs injected into the intra-articular space in an in vivo OA rabbit knee model and evaluated the effect on histological response, joint friction, and BPG distribution and retention. Rabbits underwent ACL transection to create an arthritic state after 5 weeks. OA rabbits were treated with BPGs or Euflexxa® (hyaluronic acid) intra-articular injections. Non-OA rabbits were injected similarly with BPGs; contralateral joints served as controls. The progression of OA and response to injections were evaluated using Mankin and gross grading systems indicating that mild OA was achieved in operated joints. The coefficient of friction (COF) of the intact knee joints were measured using a custom pendulum friction apparatus, showing that OA joints and OA + Euflexxa® joints demonstrated increased COF than non-operated controls, while BPG-injected non-OA and OA + BPGs were not significantly different from non-OA controls. Injected fluorescently labeled BPGs demonstrated that BPGs diffused into cartilage with localization in the pericellular region. © 2018 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 37:403-411, 2019.
(© 2018 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.)
Databáze: MEDLINE
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