Human milk oligosaccharides, milk microbiome and infant gut microbiome modulate neonatal rotavirus infection.

Autor: Ramani S; Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, 77030, TX, USA. ramani@bcm.edu., Stewart CJ; Alkek Center for Metagenomics and Microbiome Research, Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, 77030, TX, USA.; Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, NE2 4HH, UK., Laucirica DR; Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, 77030, TX, USA., Ajami NJ; Alkek Center for Metagenomics and Microbiome Research, Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, 77030, TX, USA., Robertson B; Department of Pediatrics and Larsson-Rosenquist Foundation Mother-Milk-Infant Center of Research Excellence, University of California, San Diego, La Jolla, 92093, CA, USA., Autran CA; Department of Pediatrics and Larsson-Rosenquist Foundation Mother-Milk-Infant Center of Research Excellence, University of California, San Diego, La Jolla, 92093, CA, USA., Shinge D; Division of Gastrointestinal Sciences, Christian Medical College, Vellore, 632004, India., Rani S; Division of Gastrointestinal Sciences, Christian Medical College, Vellore, 632004, India., Anandan S; Division of Gastrointestinal Sciences, Christian Medical College, Vellore, 632004, India., Hu L; Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, 77030, TX, USA., Ferreon JC; Department of Pharmacology and Chemical Biology, Baylor College of Medicine, Houston, 77030, TX, USA., Kuruvilla KA; Department of Neonatology, Christian Medical College, Vellore, 632004, India., Petrosino JF; Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, 77030, TX, USA.; Alkek Center for Metagenomics and Microbiome Research, Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, 77030, TX, USA., Venkataram Prasad BV; Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, 77030, TX, USA.; Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, 77030, TX, USA., Bode L; Department of Pediatrics and Larsson-Rosenquist Foundation Mother-Milk-Infant Center of Research Excellence, University of California, San Diego, La Jolla, 92093, CA, USA., Kang G; Division of Gastrointestinal Sciences, Christian Medical College, Vellore, 632004, India.; Translational Health Science and Technology Institute, Faridabad, 121001, India., Estes MK; Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, 77030, TX, USA.; Department of Medicine - Gastroenterology and Hepatology, Baylor College of Medicine, Houston, 77030, TX, USA.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2018 Nov 27; Vol. 9 (1), pp. 5010. Date of Electronic Publication: 2018 Nov 27.
DOI: 10.1038/s41467-018-07476-4
Abstrakt: Neonatal rotavirus infections are predominantly asymptomatic. While an association with gastrointestinal symptoms has been described in some settings, factors influencing differences in clinical presentation are not well understood. Using multidisciplinary approaches, we show that a complex interplay between human milk oligosaccharides (HMOs), milk microbiome, and infant gut microbiome impacts neonatal rotavirus infections. Validating in vitro studies where HMOs are not decoy receptors for neonatal strain G10P[11], population studies show significantly higher levels of Lacto-N-tetraose (LNT), 2'-fucosyllactose (2'FL), and 6'-siallylactose (6'SL) in milk from mothers of rotavirus-positive neonates with gastrointestinal symptoms. Further, these HMOs correlate with abundance of Enterobacter/Klebsiella in maternal milk and infant stool. Specific HMOs also improve the infectivity of a neonatal strain-derived rotavirus vaccine. This study provides molecular and translational insight into host factors influencing neonatal rotavirus infections and identifies maternal components that could promote the performance of live, attenuated rotavirus vaccines.
Databáze: MEDLINE