A novel fluorescein-bisphosphonate based diagnostic tool for the detection of hydroxyapatite in both cell and tissue models.

Autor: Sim AM; EaStCHEM School of Chemistry, University of Edinburgh, David Brewster Road, Edinburgh, EH9 3FJ, UK.; The Roslin Institute, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Midlothian, EH25 9RG, UK.; Centre for Cardiovascular Science, University of Edinburgh, Chancellor's Building, Edinburgh, EH16 4UU, UK., Rashdan NA; The Roslin Institute, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Midlothian, EH25 9RG, UK., Cui L; The Roslin Institute, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Midlothian, EH25 9RG, UK., Moss AJ; Centre for Cardiovascular Science, University of Edinburgh, Chancellor's Building, Edinburgh, EH16 4UU, UK., Nudelman F; EaStCHEM School of Chemistry, University of Edinburgh, David Brewster Road, Edinburgh, EH9 3FJ, UK., Dweck MR; Centre for Cardiovascular Science, University of Edinburgh, Chancellor's Building, Edinburgh, EH16 4UU, UK., MacRae VE; The Roslin Institute, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Midlothian, EH25 9RG, UK., Hulme AN; EaStCHEM School of Chemistry, University of Edinburgh, David Brewster Road, Edinburgh, EH9 3FJ, UK. Alison.Hulme@ed.ac.uk.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2018 Nov 26; Vol. 8 (1), pp. 17360. Date of Electronic Publication: 2018 Nov 26.
DOI: 10.1038/s41598-018-35454-9
Abstrakt: A rapid and efficient method for the detection of hydroxyapatite (HAP) has been developed which shows superiority to existing well-established methods. This fluorescein-bisphosphonate probe is highly selective for HAP over other calcium minerals and is capable of detecting lower levels of calcification in cellular models than either hydrochloric acid-based calcium leaching assays or the Alizarin S stain. The probe has been shown to be effective in both in vitro vascular calcification models and in vitro bone calcification models. Moreover we have demonstrated binding of this probe to vascular calcification in rat aorta and to areas of microcalcification, in human vascular tissue, beyond the resolution of computed tomography in human atherosclerotic plaques. Fluorescein-BP is therefore a highly sensitive and specific imaging probe for the detection of vascular calcification, with the potential to improve not only ex vivo assessments of HAP deposition but also the detection of vascular microcalcification in humans.
Databáze: MEDLINE
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