Identification of insect-specific flaviviruses in areas of Brazil and Paraguay experiencing endemic arbovirus transmission and the description of a novel flavivirus infecting Sabethes belisarioi.

Autor: Gravina HD; Laboratório de Virologia Molecular, Instituto Carlos Chagas/Fiocruz-PR, Curitiba, PR, Brazil., Suzukawa AA; Laboratório de Virologia Molecular, Instituto Carlos Chagas/Fiocruz-PR, Curitiba, PR, Brazil., Zanluca C; Laboratório de Virologia Molecular, Instituto Carlos Chagas/Fiocruz-PR, Curitiba, PR, Brazil., Cardozo Segovia FM; Departamento de Salud Pública, Instituto de Investigaciones en Ciencias de la Salud (IICS), Universidad Nacional de Asunción (UNA), Paraguay., Tschá MK; Laboratório de Virologia Molecular, Instituto Carlos Chagas/Fiocruz-PR, Curitiba, PR, Brazil., Martins da Silva A; Laboratório Central, Secretaria de Estado da Saúde (SESA), São José dos Pinhais, PR, Brazil., Faoro H; Laboratório de Regulação da Expressão Gênica (LRGEN), Instituto Carlos Chagas/Fiocruz-PR, Curitiba, PR, Brazil., da Silva Ribeiro R; Laboratório de Vigilância Ambiental, Centro de Vigilância em Saúde Ambiental (CVSA), Secretaria de Estado de Saúde (SESA), Vitória, ES, Brazil., Mendoza Torres LP; Departamento de Salud Pública, Instituto de Investigaciones en Ciencias de la Salud (IICS), Universidad Nacional de Asunción (UNA), Paraguay., Rojas A; Departamento de Salud Pública, Instituto de Investigaciones en Ciencias de la Salud (IICS), Universidad Nacional de Asunción (UNA), Paraguay., Ferrerira L; Servicio Nacional de Erradicación del Paludismo (SENEPA), Ministerio de Salud Pública y Bienestar Social, Asunción, Paraguay., Costa Ribeiro MCVD; Laboratório de Parasitologia Molecular, Universidade Federal do Paraná, Curitiba, PR, Brazil., Delfraro A; Sección Virología, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay. Electronic address: adriana@fcien.edu.uy., Duarte Dos Santos CN; Laboratório de Virologia Molecular, Instituto Carlos Chagas/Fiocruz-PR, Curitiba, PR, Brazil. Electronic address: claudia.dossantos@fiocruz.br.
Jazyk: angličtina
Zdroj: Virology [Virology] 2019 Jan 15; Vol. 527, pp. 98-106. Date of Electronic Publication: 2018 Nov 24.
DOI: 10.1016/j.virol.2018.11.008
Abstrakt: Viral infection was examined with pan-flavivirus and pan-alphavirus sets of primers in mosquitoes collected in four South American regions with confirmed pathogenic arbovirus circulation. Positive pools for flavivirus infection were sequenced and screened for specific arboviruses, which were not detected. However, NS5 gene sequencing showed that most sequences corresponded to the insect-specific Culex flavivirus. One sequence retrieved from an Aedes albopictus pool grouped with the insect-specific Aedes flavivirus and two Sabethes belisarioi pools were infected by a previously unknown flavivirus, tentatively named Sabethes flavivirus (SbFV). Phylogenetic inference placed SbFV as ancestral to a clade formed by Culiseta flavivirus, Mercadeo, and Calbertado. SbFV polyprotein showed an average aminoacidic identity of 51% in comparison to these flaviviruses. In vitro studies suggest that SbFV infects insect cells, but not vertebrate cells, therefore, we propose it as a new insect-specific flavivirus. These results highlight the wide distribution of insect-specific flaviviruses concomitant with the circulation of emergent arboviruses.
(Copyright © 2018 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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