GC-MS metabolite profiling for specific detection of dwarf somaclonal variation in banana plants.

Autor: Cevallos-Cevallos JM; Centro de Investigaciones Biotecnológicas del Ecuador Escuela Superior Politécnica del Litoral, ESPOL Campus Gustavo Galindo Km 30.5 Vía Perimetral, P.O. Box 09-01-5863 Guayaquil Ecuador.; Facultad de Ciencias de la Vida Escuela Superior Politécnica del Litoral, ESPOL Campus Gustavo Galindo Km 30.5 Vía Perimetral, P.O. Box 09-01-5863 Guayaquil Ecuador., Jines C; Centro de Investigaciones Biotecnológicas del Ecuador Escuela Superior Politécnica del Litoral, ESPOL Campus Gustavo Galindo Km 30.5 Vía Perimetral, P.O. Box 09-01-5863 Guayaquil Ecuador., Maridueña-Zavala MG; Centro de Investigaciones Biotecnológicas del Ecuador Escuela Superior Politécnica del Litoral, ESPOL Campus Gustavo Galindo Km 30.5 Vía Perimetral, P.O. Box 09-01-5863 Guayaquil Ecuador., Molina-Miranda MJ; Centro de Investigaciones Biotecnológicas del Ecuador Escuela Superior Politécnica del Litoral, ESPOL Campus Gustavo Galindo Km 30.5 Vía Perimetral, P.O. Box 09-01-5863 Guayaquil Ecuador., Ochoa DE; Facultad de Ingeniería Eléctrica y Computación Escuela Superior Politécnica del Litoral, ESPOL Campus Gustavo Galindo Km 30.5 Vía Perimetral, P.O. Box 09-01-5863 Guayaquil Ecuador., Flores-Cedeno JA; Centro de Investigaciones Biotecnológicas del Ecuador Escuela Superior Politécnica del Litoral, ESPOL Campus Gustavo Galindo Km 30.5 Vía Perimetral, P.O. Box 09-01-5863 Guayaquil Ecuador.; Facultad de Ciencias de la Vida Escuela Superior Politécnica del Litoral, ESPOL Campus Gustavo Galindo Km 30.5 Vía Perimetral, P.O. Box 09-01-5863 Guayaquil Ecuador.; Present address: Facultad de Farmacia Departamento de Biología Vegetal Universidad de Valencia 46100 Valencia Spain.
Jazyk: angličtina
Zdroj: Applications in plant sciences [Appl Plant Sci] 2018 Nov 12; Vol. 6 (11), pp. e01194. Date of Electronic Publication: 2018 Nov 12 (Print Publication: 2018).
DOI: 10.1002/aps3.1194
Abstrakt: Premise of the Study: The production of banana ( Musa spp.; Musaceae) plants is affected by various types of somaclonal variations (SV), including dwarfism. However, methods for specific detection of SV are still scarce. To overcome this, a metabolite-based method for detection of dwarf variants was evaluated.
Methods: The gas chromatography-mass spectrometry (GC-MS) metabolite profile of dwarf banana variants was investigated and compared to that of normal-healthy (N) and cucumber mosaic virus (CMV)-infected plants using principal components analysis and partial least squares discriminant analysis (PLS-DA).
Results: Significant differences among the sample groups were observed in 82 metabolites. Rhamnose was exclusively present in dwarf plants but allothreonine and trehalose were present in all but SV samples. Cellobiose was only detected in N plants, while 45 other metabolites, including methyl-glucopyranoside, allopyranose, lactose, phenylalanine, and l-lysine were detected in all but CMV-infected samples. PLS-DA models were able to detect SV, CMV, and N plants with 100% accuracy and specificity.
Discussion: The GC-MS metabolite profile can be used for the rapid, specific detection of SV at early plant production stages. This is the first metabolite-based characterization and detection of somaclonal variation in plants.
Databáze: MEDLINE
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