| Autor: |
Manatunga DC; Department of Chemistry, University of Colombo, Colombo, 00300, Sri Lanka., de Silva RM; Department of Chemistry, University of Colombo, Colombo, 00300, Sri Lanka. rohini@chem.cmb.ac.lk., de Silva KMN; Department of Chemistry, University of Colombo, Colombo, 00300, Sri Lanka.; Sri Lanka Institute of Nanotechnology (SLINTEC), Nanotechnology & Science Park, Mahenwatte, Pitipana, Homagama, 10206, Sri Lanka., Wijeratne DT; Centre for Dengue Research, Department of Microbiology, Faculty of Medical Sciences, University of Sri Jayewardenepura, Nugegoda, 10250, Sri Lanka., Malavige GN; Centre for Dengue Research, Department of Microbiology, Faculty of Medical Sciences, University of Sri Jayewardenepura, Nugegoda, 10250, Sri Lanka., Williams G; UCL School of Pharmacy, University College London, 29-39 Brunswick Square, London, WC1N 1AX, UK. |
| Abstrakt: |
Ample attention has been devoted to the construction of anti-cancer drug delivery systems with increased stability, and controlled and targeted delivery, minimizing toxic effects. In this study we have designed a magnetically attractive hydroxyapatite (m-HAP) based alginate polymer bound nanocarrier to perform targeted, controlled and pH sensitive drug release of 6-gingerol, doxorubicin, and their combination, preferably at low pH environments (pH 5.3). They have exhibited higher encapsulation efficiency which is in the range of 97.4-98.9% for both 6-gingerol and doxorubicin molecules whereas the co-loading has accounted for a value of 81.87 ± 0.32%. Cell proliferation assays, fluorescence imaging and flow cytometric analysis, demonstrated the remarkable time and dose responsive anti-proliferative effect of drug loaded nanoparticles on MCF-7 cells and HEpG2 cells compared with their neat counter parts. Also, these systems have exhibited significantly reduced toxic effects on non-targeted, non-cancerous cells in contrast to the excellent ability to selectively kill cancerous cells. This study has suggested that this HAP based system is a versatile carrier capable of loading various drug molecules, ultimately producing a profound anti-proliferative effect. |
