Impact of nanoparticles on transcriptional regulation of catabolic genes of petroleum hydrocarbon-degrading bacteria in contaminated soil microcosms.

Autor: El-Sayed WS; Biology Department, Faculty of Science, Taibah University, Almadinah Almunawarah, Saudi Arabia.; Microbiology Department, Faculty of Science, Ain Shams University, Cairo, Egypt., Elbahloul Y; Biology Department, Faculty of Science, Taibah University, Almadinah Almunawarah, Saudi Arabia.; Botany and Microbiology Department, Faculty of Science, Alexandria University, Alexandria, Egypt., Saad ME; Biology Department, Faculty of Science, Taibah University, Almadinah Almunawarah, Saudi Arabia.; Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center (ARC), Giza, Egypt., Hanafy AM; Biology Department, Faculty of Science, Taibah University, Almadinah Almunawarah, Saudi Arabia.; Microbiology Department, Faculty of Science, Ain Shams University, Cairo, Egypt., Hegazi AH; Chemistry Department, Faculty of Science, Taibah University, Almadinah Almunawarah, Saudi Arabia.; Chemistry Department, Faculty of Science, Alexandria University, Alexandria, Egypt., ElShafei GMS; Chemistry Department, Faculty of Science, Taibah University, Almadinah Almunawarah, Saudi Arabia.; Chemistry Department, Faculty of Science, Ain Shams University, Cairo, Egypt., Elbadry M; Biology Department, Faculty of Science, Taibah University, Almadinah Almunawarah, Saudi Arabia.; Agricultural Microbiology Department, Faculty of Agriculture, Fayoum University, Fayoum, Egypt.
Jazyk: angličtina
Zdroj: Journal of basic microbiology [J Basic Microbiol] 2019 Feb; Vol. 59 (2), pp. 166-180. Date of Electronic Publication: 2018 Nov 23.
DOI: 10.1002/jobm.201800186
Abstrakt: This study was conducted to determine what effects nanoparticles (NPs) like TiO 2 , ZnO, and Ag may pose on natural attenuation processes of petroleum hydrocarbons in contaminated soils. The solid NPs used were identified using x-ray diffraction technique and their average size was certified as 18.2, 16.9, and 18.3 nm for Ag-NPs, ZnO-NPs, and TiO 2 -NPs, respectively. NPs in soil microcosms behave differently where it was dissolved as in case of Ag-NPs, partially dissolved as in ZnO-NPs or changed into other crystalline phase as in TiO 2 -NPs. In this investigation, catabolic gene encoding catechol 2,3 dioxygenase (C23DO) was selected specifically as biomarker for monitoring hydrocarbon biodegradation potential by measuring its transcripts by RT-qPCR. TiO 2 -NPs amended microcosms showed almost no change in C23DO expression profile or bacterial community which were dominated by Bacillus sp., Mycobacterium sp., Microbacterium sp., Clostridium sp., beside uncultured bacteria, including uncultured proteobacteria, Thauera sp. and Clostridia. XRD pattern suggested that TiO 2 -NPs in microcosms were changed into other non-inhibitory crystalline phase, consequently, showing the maximum degradation profile for most low molecular weight oil fractions and partially for the high molecular weight ones. Increasing ZnO-NPs concentration in microcosms resulted in a reduction in the expression of C23DO with a concomitant slight deteriorative effect on bacterial populations ending up with elimination of Clostridium sp., Thauera sp., and uncultured proteobacteria. The oil-degradation efficiency was reduced compared to TiO 2 -NPs amended microcosms. In microcosms, Ag-NPs were not detected in the crystalline form but were available in the ionic form that inhibited most bacterial populations and resulted in a limited degradation profile of oil, specifically the low molecular weight fractions. Ag-NPs amended microcosms showed a significant reduction (80%) in C23DO gene expression and a detrimental effect on bacterial populations including key players like Mycobacterium sp., Microbacterium sp., and Thauera sp. involved in the biodegradation of petroleum hydrocarbons.
(© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.)
Databáze: MEDLINE
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