Molecular Factors of Hypochlorite Tolerance in the Hypersaline Archaeon Haloferax volcanii.

Autor:	Gomez M; Department of Microbiology and Cell Science, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611, USA. migmez10@gmail.com., Leung W; Department of Microbiology and Cell Science, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611, USA. whinkie1388@gmail.com., Dantuluri S; Department of Microbiology and Cell Science, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611, USA. swathidantuluri@ufl.edu., Pillai A; Department of Microbiology and Cell Science, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611, USA. pillai.alexander@gmail.com., Gani Z; Department of Microbiology and Cell Science, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611, USA. z.gani1997@ufl.edu., Hwang S; Department of Microbiology and Cell Science, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611, USA. sungmin.hwang@duke.edu., McMillan LJ; Department of Microbiology and Cell Science, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611, USA. lana.mcmillan@locus-bio.com.; Genetics Institute, University of Florida, Gainesville, FL 32611, USA. lana.mcmillan@locus-bio.com., Kiljunen S; Department of Bacteriology and Immunology, Immunobiology Research Program, University of Helsinki, 00014 Helsinki, Finland. saija.kiljunen@helsinki.fi., Savilahti H; Division of Genetics and Physiology, Department of Biology, University of Turku, 20014 Turku, Finland. harri.savilahti@utu.fi., Maupin-Furlow JA; Department of Microbiology and Cell Science, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32611, USA. jmaupin@ufl.edu.; Genetics Institute, University of Florida, Gainesville, FL 32611, USA. jmaupin@ufl.edu.
Jazyk:	angličtina
Zdroj:	Genes [Genes (Basel)] 2018 Nov 20; Vol. 9 (11). Date of Electronic Publication: 2018 Nov 20.
DOI:	10.3390/genes9110562
Abstrakt:	Halophilic archaea thrive in hypersaline conditions associated with desiccation, ultraviolet (UV) irradiation and redox active compounds, and thus are naturally tolerant to a variety of stresses. Here, we identified mutations that promote enhanced tolerance of halophilic archaea to redox-active compounds using Haloferax volcanii as a model organism. The strains were isolated from a library of random transposon mutants for growth on high doses of sodium hypochlorite (NaOCl), an agent that forms hypochlorous acid (HOCl) and other redox acid compounds common to aqueous environments of high concentrations of chloride. The transposon insertion site in each of twenty isolated clones was mapped using the following: (i) inverse nested two-step PCR (INT-PCR) and (ii) semi-random two-step PCR (ST-PCR). Genes that were found to be disrupted in hypertolerant strains were associated with lysine deacetylation, proteasomes, transporters, polyamine biosynthesis, electron transfer, and other cellular processes. Further analysis revealed a Δ psmA1 (α1) markerless deletion strain that produces only the α2 and β proteins of 20S proteasomes was hypertolerant to hypochlorite stress compared with wild type, which produces α1, α2, and β proteins. The results of this study provide new insights into archaeal tolerance of redox active compounds such as hypochlorite. Competing Interests: The authors do not have a conflict of interest to declare.
Databáze:	MEDLINE
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