| Autor: |
Costa CMS; Programa de Pós-graduação em Aquicultura, Universidade Nilton Lins, Manaus, AM, Brasil., Lima TBC; Programa de Pós-graduação em Aquicultura, Universidade Nilton Lins, Manaus, AM, Brasil., Cruz MGD; Programa de Pós-graduação em Aquicultura, Universidade Nilton Lins, Manaus, AM, Brasil., Almeida DV; Programa de Pós-graduação em Aquicultura, Universidade Nilton Lins, Manaus, AM, Brasil., Martins ML; Laboratório Sanidade de Organismos Aquáticos - AQUOS, Departamento de Aquicultura, Universidade Federal de Santa Catarina - UFSC, Florianópolis, SC, Brasil., Jerônimo GT; Laboratório Sanidade de Organismos Aquáticos - AQUOS, Departamento de Aquicultura, Universidade Federal de Santa Catarina - UFSC, Florianópolis, SC, Brasil. |
| Abstrakt: |
Infection by the acantocephalan Neoechinorhynchus buttnerae is considered one of most important concerns for tambaqui fish (Colossoma macropomum ) production. Treatment strategies have been the focus of several in vivo studies; however, few studies have been undertaken on in vitro protocols for parasite maintenance. The aim of the present study was to develop the best in vitro culture condition for N. buttnerae to ensure its survival and adaptation out of the host to allow for the testing of substances to be used to control the parasite. To achieve this, parasites were collected from naturally infected fish and distributed in 6-well culture plates under the following treatments in triplicate: 0.9% NaCl, sterile tank water, L-15 Leibovitz culture medium, L-15 Leibovitz + agar 2% culture medium, RPMI 1640 culture medium, and RPMI 1640 + agar 2% culture medium. The plates containing the parasites were maintained at 24 °C, 28 °C, and 32 °C. The RPMI 1640 + agar 2% culture medium showed the best survival of 24 days at 24 °C. No body alterations such as swollen parasites, body deformation, dehydration and hardening were observed in the RPMI 1640 + 2% culture medium. |
