Effect of eccentric action velocity on expression of genes related to myostatin signaling pathway in human skeletal muscle.
| Autor: | Roschel H; School of Physical Education and Sport, University of São Paulo, São Paulo, SP, Brazil., Ugrinowistch C; School of Physical Education and Sport, University of São Paulo, São Paulo, SP, Brazil., Santos AR; School of Arts, Sciences and Humanities, University of São Paulo, São Paulo, SP, Brazil., Barbosa WP; School of Physical Education and Sport, University of São Paulo, São Paulo, SP, Brazil., Miyabara EH; Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP, Brazil., Tricoli V; School of Physical Education and Sport, University of São Paulo, São Paulo, SP, Brazil., Aoki MS; School of Arts, Sciences and Humanities, University of São Paulo, São Paulo, SP, Brazil. |
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| Jazyk: | angličtina |
| Zdroj: | Biology of sport [Biol Sport] 2018 Jun; Vol. 35 (2), pp. 111-119. Date of Electronic Publication: 2017 Nov 23. |
| DOI: | 10.5114/biolsport.2018.71600 |
| Abstrakt: | The aim of this study was to investigate the effects of an acute bout of eccentric actions, performed at fast velocity (210º . s -1 ) and at slow velocity (20º . s -1 ), on the gene expression of regulatory components of the myostatin (MSTN) signalling pathway. Participants performed an acute bout of eccentric actions at either a slow or a fast velocity. Muscle biopsy samples were taken before, immediately after, and 2 h after the exercise bout. The gene expression of the components of the MSTN pathway was assessed by real-time PCR. No change was observed in MSTN, ACTRIIB, GASP-1 or FOXO-3a gene expression after either slow or fast eccentric actions (p > 0.05). However, the MSTN inhibitors follistatin (FST), FST-like-3 (FSTL3) and SMAD-7 were significantly increased 2 h after both eccentric actions (p < 0.05). No significant difference between bouts was found before, immediately after, or 2 h after the eccentric actions (slow and fast velocities, p > 0.05). The current findings indicate that a bout of eccentric actions activates the expression of MSTN inhibitors. However, no difference was observed in MSTN inhibitors' gene expression when comparing slow and fast eccentric actions. It is possible that the greater time under tension induced by slow eccentric (SE) actions might compensate the effect of the greater velocity of fast eccentric (FE) actions. Additional studies are required to address the effect of eccentric action (EA) velocities on the pathways related to muscle hypertrophy. Competing Interests: The authors declare that there is no conflict of interests regarding the publication of this paper. |
| Databáze: | MEDLINE |
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