Effect of immobilization on the activity of catalase carried by poly(HEMA-GMA) cryogels.

Autor: Erol K; Osmancik Omer Derindere Vocational School, Department of Property Protection and Safety, Hitit University, Corum 19500, Turkey., Cebeci BK; Alaca Avni Celik Vocational School, Department of Food Processing, Hitit University, Corum 19600, Turkey., Köse K; Alaca Avni Celik Vocational School, Department of Food Processing, Hitit University, Corum 19600, Turkey. Electronic address: kazimkose@hitit.edu.tr., Köse DA; Faculty of Science and Arts, Department of Molecular Biology and Genetics, Hitit University, Corum, 19040, Turkey.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2019 Feb 15; Vol. 123, pp. 738-743. Date of Electronic Publication: 2018 Nov 16.
DOI: 10.1016/j.ijbiomac.2018.11.121
Abstrakt: Hydrogen peroxide is converted by catalase to molecular oxygen and water to remove oxidative stress. In this study, catalase immobilization was performed using poly(2-hydroxyethyl methacrylate-glycidyl methacrylate) (poly(HEMA-GMA)) cryogels with different amounts of GMA. Catalase adsorption capacity of 298.7 ± 9.9 mg/g was achieved at the end of 9 h using the poly(HEMA-GMA)-250 cryogel. Kinetic parameters and the inhibitory effects of pesticides such as 4,4'-DDE and 4,4'-DDT on the activity of free and immobilized catalase enzyme were investigated. While the V max value of the immobilized enzyme was reduced 4-fold compared to the free enzyme, in the case of the comparison of the K M values, the affinity of the immobilized enzyme was increased by 1.94 times against the substrate. The inhibitory effect of 4,4'-DDT pesticide was found to be higher for the immobilized and free enzyme. NaCl (1 M, pH: 7.0) solution was used for desorption of the adsorbed catalase enzyme. A desorption ratio of 96.45% was achieved. The technique used in this study is promising regarding for the immobilization of catalase enzyme to increase the operational activity. Therefore, poly(HEMA-GMA) cryogels have the potential to be used for immobilization of catalase enzyme in the fields of biology and biochemistry.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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