Peculiar features of the plastids of the colourless alga Euglena longa and photosynthetic euglenophytes unveiled by transcriptome analyses.

Autor: Záhonová K; Life Science Research Centre, Department of Biology and Ecology and Institute of Environmental Technologies, Faculty of Science, University of Ostrava, 701 00, Ostrava, Czech Republic., Füssy Z; Institute of Parasitology, Biology Centre CAS, 370 05, České Budějovice, Czech Republic., Birčák E; Department of Genetics, Faculty of Natural Sciences, Comenius University, 842 15, Bratislava, Slovakia., Novák Vanclová AMG; Department of Parasitology, Faculty of Science, Charles University, BIOCEV, Prague, Czech Republic., Klimeš V; Life Science Research Centre, Department of Biology and Ecology and Institute of Environmental Technologies, Faculty of Science, University of Ostrava, 701 00, Ostrava, Czech Republic., Vesteg M; Department of Biology and Ecology, Faculty of Natural Sciences, Matej Bel University, 974 01, Banská Bystrica, Slovakia., Krajčovič J; Department of Biology, Faculty of Natural Sciences, University of ss. Cyril and Methodius in Trnava, 917 01, Trnava, Slovakia., Oborník M; Institute of Parasitology, Biology Centre CAS, 370 05, České Budějovice, Czech Republic.; University of South Bohemia, Faculty of Science, 370 05, České Budějovice, Czech Republic., Eliáš M; Life Science Research Centre, Department of Biology and Ecology and Institute of Environmental Technologies, Faculty of Science, University of Ostrava, 701 00, Ostrava, Czech Republic. marek.elias@osu.cz.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2018 Nov 19; Vol. 8 (1), pp. 17012. Date of Electronic Publication: 2018 Nov 19.
DOI: 10.1038/s41598-018-35389-1
Abstrakt: Euglenophytes are a familiar algal group with green alga-derived secondary plastids, but the knowledge of euglenophyte plastid function and evolution is still highly incomplete. With this in mind we sequenced and analysed the transcriptome of the non-photosynthetic species Euglena longa. The transcriptomic data confirmed the absence of genes for the photosynthetic machinery, but provided candidate plastid-localised proteins bearing N-terminal bipartite topogenic signals (BTSs) of the characteristic euglenophyte type. Further comparative analyses including transcriptome assemblies available for photosynthetic euglenophytes enabled us to unveil salient aspects of the basic euglenophyte plastid infrastructure, such as plastidial targeting of several proteins as C-terminal translational fusions with other BTS-bearing proteins or replacement of the conventional eubacteria-derived plastidial ribosomal protein L24 by homologs of archaeo-eukaryotic origin. Strikingly, no homologs of any key component of the TOC/TIC system and the plastid division apparatus are discernible in euglenophytes, and the machinery for intraplastidial protein targeting has been simplified by the loss of the cpSRP/cpFtsY system and the SEC2 translocon. Lastly, euglenophytes proved to encode a plastid-targeted homolog of the termination factor Rho horizontally acquired from a Lambdaproteobacteria-related donor. Our study thus further documents a substantial remodelling of the euglenophyte plastid compared to its green algal progenitor.
Databáze: MEDLINE
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