Protective effect of Myrsine parvifolia plant extract against the inflammatory process induced by Bothrops jararaca snake venom.

Autor: Corrêa AL; Laboratório de Tecnologia de Produtos Naturais, Departamento de Tecnologia Farmacêutica, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil. Electronic address: arthur_farm@yahoo.com.br., Oliveira AP; Departamento de Fármacos e Medicamentos, Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil. Electronic address: adrianapassos@pharma.ufrj.br., Ruppelt BM; Laboratório de Tecnologia de Produtos Naturais, Departamento de Tecnologia Farmacêutica, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil., de Araújo ERA; Laboratório de Tecnologia de Produtos Naturais, Departamento de Tecnologia Farmacêutica, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil., Santos MG; Departamento de Ciências, Faculdade de Formação de Professores, Universidade, Estadual do Rio de Janeiro, São Gonçalo, Rio de Janeiro, Brazil., Caldas GR; Laboratório de Tecnologia de Produtos Naturais, Departamento de Tecnologia Farmacêutica, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil., Muylaert FF; Laboratório de Farmacologia, Departamento de Farmacologia e Toxicologia, Instituto Nacional de Controle de Qualidade em Saúde, Fundação Oswaldo Cruz, Rio de Janeiro, Rio de Janeiro, Brazil., Amendoeira FC; Laboratório de Farmacologia, Departamento de Farmacologia e Toxicologia, Instituto Nacional de Controle de Qualidade em Saúde, Fundação Oswaldo Cruz, Rio de Janeiro, Rio de Janeiro, Brazil., Ferraris FK; Laboratório de Farmacologia, Departamento de Farmacologia e Toxicologia, Instituto Nacional de Controle de Qualidade em Saúde, Fundação Oswaldo Cruz, Rio de Janeiro, Rio de Janeiro, Brazil., de Souza CMV; Laboratório de Artrópodos, Instituto Vital Brazil, Niterói, Rio de Janeiro, Brazil., Fuly AL; Instituto de Biologia, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil., Rocha L; Laboratório de Tecnologia de Produtos Naturais, Departamento de Tecnologia Farmacêutica, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil.
Jazyk: angličtina
Zdroj: Toxicon : official journal of the International Society on Toxinology [Toxicon] 2019 Jan; Vol. 157, pp. 66-76. Date of Electronic Publication: 2018 Nov 14.
DOI: 10.1016/j.toxicon.2018.11.289
Abstrakt: Accidents involving snakes from the genus Bothrops sp. constitute the most important cause of snake envenomation in Brazil. The Myrsine genus has been reported to be used in folk medicine against snakebites. In this work, the phytochemical profiles and ability of extracts from Myrsine parvifolia leaves to reduce the inflammatory process (edema, vascular permeability increase and leukocyte migration) induced by Bothrops jararaca venom were investigated in vivo. Chemical compounds were identified by chromatographic and spectroscopy techniques. Total polyphenol, tannin, and flavonoid contents were determined by spectrophotometric methods. Swiss male mice received an oral administration of extracts (100 mg/kg) in different protocols. Paw edema, intraperitoneal vascular permeability and pleurisy models in mice were used to evaluate the antiophidic potential of the extracts. Paw edema was induced by subplantar injection of B. jararaca venom and quantified as the increase in paw volume. Changes in vascular permeability were assessed by measuring the amount of Evans blue dye extravasation. Leukocyte migration was assessed by total and differential counts in the pleural cavity washes. Myricetin, myricetin-3-O-β-arabinopyranoside, quercetin and kaempferol were isolated from the ethyl acetate extract and identified as the primary compounds of the dichloromethane extract. Terpenes and fatty acids were identified in the hexane and dichloromethane extracts. The pretreated group with hydroethanolic and dichloromethane extract reduced total edema (40 and 52%, respectively), vascular permeability increase (32.4 and 32.2%, respectively) and leukocyte influx into the pleural cavity (42 and 39%, respectively), while the group treated with hexane extract showed only reduced edema (37%) induced by B. jararaca venom. The hydroethanolic extract showed better results in all of the tests performed and was also administered by the protocol of post-poisoning, showing maintenance of paw edema reduction and cell migration. These data indicate a potential anti-inflammatory activity of M. parvifolia in poisoning by B. jararaca, especially to reduce local poison effects.
(Copyright © 2018 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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