Temperature-dependent vitamin D signaling regulates developmental trajectory associated with diapause in an annual killifish.

Autor: Romney ALT; Department of Biology, Portland State University, Portland, OR 97207.; School of Veterinary Medicine, Anatomy, Physiology, and Cell Biology, University of California, Davis, CA 95616., Davis EM; Department of Biology, Portland State University, Portland, OR 97207., Corona MM; Department of Biology, Portland State University, Portland, OR 97207., Wagner JT; Knight Cancer Early Detection Advanced Research Center, Oregon Health and Science University, Portland, OR 97201., Podrabsky JE; Department of Biology, Portland State University, Portland, OR 97207; jpod@pdx.edu.
Jazyk: angličtina
Zdroj: Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2018 Dec 11; Vol. 115 (50), pp. 12763-12768. Date of Electronic Publication: 2018 Nov 16.
DOI: 10.1073/pnas.1804590115
Abstrakt: The mechanisms that integrate environmental signals into developmental programs remain largely uncharacterized. Nuclear receptors (NRs) are ligand-regulated transcription factors that orchestrate the expression of complex phenotypes. The vitamin D receptor (VDR) is an NR activated by 1α,25-dihydroxyvitamin D 3 [1,25(OH) 2 D 3 ], a hormone derived from 7-dehydrocholesterol (7-DHC). VDR signaling is best known for regulating calcium homeostasis in mammals, but recent evidence suggests a diversity of uncharacterized roles. In response to incubation temperature, embryos of the annual killifish Austrofundulus limnaeus can develop along two alternative trajectories: active development and diapause. These trajectories diverge early in development, from a biochemical, morphological, and physiological perspective. We manipulated incubation temperature to induce the two trajectories and profiled changes in gene expression using RNA sequencing and weighted gene coexpression network analysis. We report that transcripts involved in 1,25(OH) 2 D 3 synthesis and signaling are expressed in a trajectory-specific manner. Furthermore, exposure of embryos to vitamin D 3 analogs and Δ4-dafachronic acid directs continuous development under diapause-inducing conditions. Conversely, blocking synthesis of 1,25(OH) 2 D 3 induces diapause in A. limnaeus and a diapause-like state in zebrafish, suggesting vitamin D signaling is critical for normal vertebrate development. These data support vitamin D signaling as a molecular pathway that can regulate developmental trajectory and metabolic dormancy in a vertebrate. Interestingly, the VDR is homologous to the daf-12 and ecdysone NRs that regulate dormancy in Caenorhabditis elegans and Drosophila We suggest that 7-DHC-derived hormones and their associated NRs represent a conserved pathway for the integration of environmental information into developmental programs associated with life history transitions in animals.
Competing Interests: The authors declare no conflict of interest.
Databáze: MEDLINE
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