Protective effects of neocuproine copper chelator against oxidative damage in NSC34 cells.

Autor: Nunes EA; Center for Natural Sciences and Humanities, Federal University of ABC - UFABC, Avenida dos Estados, 5001, Bloco B, 09210-580, Santo André, SP, Brazil., Manieri TM; Center for Natural Sciences and Humanities, Federal University of ABC - UFABC, Avenida dos Estados, 5001, Bloco B, 09210-580, Santo André, SP, Brazil., Matias AC; Center for Natural Sciences and Humanities, Federal University of ABC - UFABC, Avenida dos Estados, 5001, Bloco B, 09210-580, Santo André, SP, Brazil., Bertuchi FR; Center for Natural Sciences and Humanities, Federal University of ABC - UFABC, Avenida dos Estados, 5001, Bloco B, 09210-580, Santo André, SP, Brazil., da Silva DA; Center for Natural Sciences and Humanities, Federal University of ABC - UFABC, Avenida dos Estados, 5001, Bloco B, 09210-580, Santo André, SP, Brazil., Lago L; Center for Natural Sciences and Humanities, Federal University of ABC - UFABC, Avenida dos Estados, 5001, Bloco B, 09210-580, Santo André, SP, Brazil., Sato RH; Center for Natural Sciences and Humanities, Federal University of ABC - UFABC, Avenida dos Estados, 5001, Bloco B, 09210-580, Santo André, SP, Brazil., Cerchiaro G; Center for Natural Sciences and Humanities, Federal University of ABC - UFABC, Avenida dos Estados, 5001, Bloco B, 09210-580, Santo André, SP, Brazil. Electronic address: giselle.cerchiaro@ufabc.edu.br.
Jazyk: angličtina
Zdroj: Mutation research. Genetic toxicology and environmental mutagenesis [Mutat Res Genet Toxicol Environ Mutagen] 2018 Dec; Vol. 836 (Pt B), pp. 62-71. Date of Electronic Publication: 2018 Jun 25.
DOI: 10.1016/j.mrgentox.2018.06.019
Abstrakt: In this work, we aim to provide evidence for the protective effect of a copper chelator, neocuproine (NeoCu), against the oxidative stress in NSC34 cells, which inhibits biomolecule oxidation and cell death. Results obtained with the comet assay allowed to determine the increase in oxidized purines and pyrimidines by H 2 O 2 exposure, and their changes after the addition of NeoCu. We also observed a higher ATP7b activity in nuclei and a higher Cu concentration inside the cells, proving that the NeoCu acts directly in DNA to promote cell recovery in oxidative stress conditions, also observed in Reactive Oxygen Species (ROS) detection assay by Flow Cytometry. Based on these results, we propose that NeoCu is a promising drug for the protection of motor neuron cells during oxidative stress caused by neurodegenerative diseases in this system.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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