Amido Black 10B a widely used azo dye causes DNA damage in pro- and eukaryotic indicator cells.

Autor: Carvalho da Cruz Brambilla CM; Laboratory of Genetic Toxicology, PPGBioSaúde and PPGGTA, Lutheran University of Brazil (ULBRA), Av. Farroupilha 8001, Prédio 22 (4º Andar) 92425-900, Canoas, RS, Brazil., Hilario Garcia AL; Laboratory of Genetic Toxicology, PPGBioSaúde and PPGGTA, Lutheran University of Brazil (ULBRA), Av. Farroupilha 8001, Prédio 22 (4º Andar) 92425-900, Canoas, RS, Brazil; Laboratory of Ecotoxicology, Posgraduate Progam in Enviromental Quality, University Feevale, ERS-239, 2755, 93525-075, Novo Hamburgo, RS, Brazil., Rabaioli da Silva F; La Salle University, Masteŕs Degree in Environmental Impact Evaluation, Canoas, RS, Brazil., Taffarel SR; La Salle University, Masteŕs Degree in Environmental Impact Evaluation, Canoas, RS, Brazil., Grivicich I; Laboratory of Cancer Biology, PPGBioSaúde, Lutheran University of Brazil (ULBRA), Av. Farroupilha 8001, Prédio 22 (5º Andar) 92425-900, Canoas, RS, Brazil., Picada JN; Laboratory of Genetic Toxicology, PPGBioSaúde and PPGGTA, Lutheran University of Brazil (ULBRA), Av. Farroupilha 8001, Prédio 22 (4º Andar) 92425-900, Canoas, RS, Brazil., Scotti A; Laboratory of Genetic Toxicology, PPGBioSaúde and PPGGTA, Lutheran University of Brazil (ULBRA), Av. Farroupilha 8001, Prédio 22 (4º Andar) 92425-900, Canoas, RS, Brazil., Dalberto D; Laboratory of Genetic Toxicology, PPGBioSaúde and PPGGTA, Lutheran University of Brazil (ULBRA), Av. Farroupilha 8001, Prédio 22 (4º Andar) 92425-900, Canoas, RS, Brazil., Mišík M; Institute of Cancer Research, Department of Internal Medicine I, Medical University of Vienna, Borschkegasse 8a, A-1090 Vienna, Austria., Knasmüller S; Institute of Cancer Research, Department of Internal Medicine I, Medical University of Vienna, Borschkegasse 8a, A-1090 Vienna, Austria., da Silva J; Laboratory of Genetic Toxicology, PPGBioSaúde and PPGGTA, Lutheran University of Brazil (ULBRA), Av. Farroupilha 8001, Prédio 22 (4º Andar) 92425-900, Canoas, RS, Brazil. Electronic address: juliana.silva@ulbra.br.
Jazyk: angličtina
Zdroj: Chemosphere [Chemosphere] 2019 Feb; Vol. 217, pp. 430-436. Date of Electronic Publication: 2018 Nov 03.
DOI: 10.1016/j.chemosphere.2018.11.026
Abstrakt: Acid Black 10B (AB10B) is widely used for the production of textiles, leather and prints. It is a representative of azo dyes and it is well documented that some of these compounds are mutagenic per se, and that cleavage products (in particular aromatic amines) may cause damage of the genetic material and cancer. Since no toxicological data on AB10B have been published, we evaluated its mutagenic activity in Salmonella/microsome assays and studied its acute toxic and genotoxic properties in a human derived liver cell line (HepG2) which retained the activities of drug metabolizing enzymes. The compound did not cause cytotoxicity (MTT assay), but clear genotoxic effects were detected in pro- and eukaryotic indicator cells. Dose dependent induction of his + revertants was seen in strain TA98 which detects frameshift mutations without metabolic activation; a more pronounced effect was seen in its derivative YG1024 which overexpresses N-acetyltransferase. Induction of single/double strand breaks by Comet assay was detected with concentrations > 0.125 mg/mL in liver derived cells; as well as increased rates for micronucleus (reflecting structural and numeric chromosomal aberrations) and nuclear buds which are a consequence of gene amplifications were seen with a higher dose (2.0 mg/mL) (p < 0.05; Tukey's test). The mutational pattern which was observed in the bacterial tests indicates that the cleavage product p-nitroaniline may cause the genotoxic effects of the dye. Our findings indicate that exposure of humans and the release of the compound into the environment may lead to adverse effects due to its DNA damaging activity.
(Copyright © 2018 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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