Silk Molecular Weight Influences the Kinetics of Enzymatically Cross-linked Silk Hydrogel Formation.

Autor:	Tabatabai AP; Department of Physics , Georgetown University , Washington , District of Columbia 20057 , United States.; Institute for Soft Matter Synthesis and Metrology , Georgetown University , Washington , District of Columbia 20057 , United States., Partlow BP; Department of Biomedical Engineering , Tufts University , Medford , Massachusetts 02155 , United States., Raia NR; Department of Biomedical Engineering , Tufts University , Medford , Massachusetts 02155 , United States., Kaplan DL; Department of Biomedical Engineering , Tufts University , Medford , Massachusetts 02155 , United States., Blair DL; Department of Physics , Georgetown University , Washington , District of Columbia 20057 , United States.; Institute for Soft Matter Synthesis and Metrology , Georgetown University , Washington , District of Columbia 20057 , United States.
Jazyk:	angličtina
Zdroj:	Langmuir : the ACS journal of surfaces and colloids [Langmuir] 2018 Dec 18; Vol. 34 (50), pp. 15383-15387. Date of Electronic Publication: 2018 Nov 27.
DOI:	10.1021/acs.langmuir.8b02950
Abstrakt:	We transform reconstituted silk solutions into robust hydrogels through covalent dityrosine cross-linking resulting from an enzymatic reaction. The bulk rheological properties and the covalent dityrosine bond formation of these gels are measured during polymerization. We compare the time-resolved bond formation to the mechanical properties, where we find that the gelation process is consistent with a model of percolation. The molecular weight of the protein determines whether a secondary mode of growth postpercolation exists, indicating that molecular weight changes affect the mechanisms by which these gels polymerize.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu