Validation of the blood gas analyzer for pH measurements in IVF culture medium: Prevent suboptimal culture conditions.

Autor: Diaz de Pool JDN; Department of Gynecology, Leiden University Medical Center, Leiden, The Netherlands., Van Den Berg SAA; Department of Clinical Chemistry, Leiden University Medical Center, Leiden, The Netherlands., Pilgram GSK; Department of Gynecology, Leiden University Medical Center, Leiden, The Netherlands., Ballieux BEPB; Department of Clinical Chemistry, Leiden University Medical Center, Leiden, The Netherlands., Van Der Westerlaken LAJ; Department of Gynecology, Leiden University Medical Center, Leiden, The Netherlands.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2018 Nov 12; Vol. 13 (11), pp. e0206707. Date of Electronic Publication: 2018 Nov 12 (Print Publication: 2018).
DOI: 10.1371/journal.pone.0206707
Abstrakt: Measurement of pH in IVF-media using the blood gas analyzer (BGA) requires validation, because IVF-media is outside the intended scope of the BGA. To determine whether the Siemens Rapidpoint 500 BGA is suitable for pH measurements in IVF-media this study will validate the BGA and assess its accuracy. In this method comparison study, the pH of over three hundred IVF-media samples was measured with the BGA and a pH electrode (Hanna pH checker). The precision of both the BGA and the pH electrode were excellent (coefficient variation <1.4%). However, the closeness of agreement between measured values of both devices were not equivalent to each other in the tested IVF-media, showing 15% to 85% accordance between devices. The pH measured with the blood gas analyzer was also significantly higher in the tested media, compared to that measured by the pH electrode. One of the tested media did not reach its target pH when it was measured with the BGA, even at 9% CO2. The results show that the validated blood gas analyzer produces excellent results in terms of precision but not in terms of accuracy. Inaccurate measurement may lead to misinterpretation of results and consequently to suboptimal culture conditions. Therefore, each laboratory is encouraged to perform a validation of their BGA.
Competing Interests: The authors have declared that no competing interests exist.
Databáze: MEDLINE
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