| Autor: |
Martins PK; Genetics and Biotechnology Laboratory, Embrapa Agroenergy (CNPAE), Brazilian Agricultural Research Corporation (EMBRAPA), Parque Estação Biológica, Brasília, DF, Brazil., da Cunha BADB; Genetics and Biotechnology Laboratory, Embrapa Agroenergy (CNPAE), Brazilian Agricultural Research Corporation (EMBRAPA), Parque Estação Biológica, Brasília, DF, Brazil., Kobayshi AK; Genetics and Biotechnology Laboratory, Embrapa Agroenergy (CNPAE), Brazilian Agricultural Research Corporation (EMBRAPA), Parque Estação Biológica, Brasília, DF, Brazil., Molinari HBC; Genetics and Biotechnology Laboratory, Embrapa Agroenergy (CNPAE), Brazilian Agricultural Research Corporation (EMBRAPA), Parque Estação Biológica, Brasília, DF, Brazil. hugo.molinari@embrapa.br. |
| Abstrakt: |
Setaria viridis is an emerging model for C4 species, and it is an important model to validate some genes for further C4 crop transformation, such as sugarcane, maize, and wheat. Here, we describe two protocols for stable transformation of S. viridis mediated by Agrobacterium tumefaciens with three different reporter genes and two selectable markers. Routine transformation efficiency reaching 29% was achieved using embryogenic callus in S. viridis (accession A10.1). Alternatively, we developed a transformation method by floral dip with 0.6% efficiency. The developed protocols could be useful for genetic and genomics studies of important food-feed-fiber-fuel C4 crops. |
