(Pro)renin/renin receptor expression during normal and preeclamptic pregnancy in rats.

Autor: Avila-Ramírez MA; Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina del IPN, D.F., México, Plan de San Luis y Díaz Mirón, Casco de Santo Tomás, México 11340 D.F., Mexico., Esteban-Martínez RL; Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina del IPN, D.F., México, Plan de San Luis y Díaz Mirón, Casco de Santo Tomás, México 11340 D.F., Mexico., López-Moctezuma E; Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina del IPN, D.F., México, Plan de San Luis y Díaz Mirón, Casco de Santo Tomás, México 11340 D.F., Mexico., Anguiano-Robledo L; Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina del IPN, D.F., México, Plan de San Luis y Díaz Mirón, Casco de Santo Tomás, México 11340 D.F., Mexico., Hernández-Campos ME; Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina del IPN, D.F., México, Plan de San Luis y Díaz Mirón, Casco de Santo Tomás, México 11340 D.F., Mexico., López-Sánchez P; Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina del IPN, D.F., México, Plan de San Luis y Díaz Mirón, Casco de Santo Tomás, México 11340 D.F., Mexico. Electronic address: pelosa651018@yahoo.com.
Jazyk: angličtina
Zdroj: Life sciences [Life Sci] 2019 Jan 01; Vol. 216, pp. 22-28. Date of Electronic Publication: 2018 Nov 08.
DOI: 10.1016/j.lfs.2018.11.017
Abstrakt: Aims: Pregnancy is a physiological stage with profound cardiovascular changes leading to hypotension. Preeclampsia (PE) reverts these normal changes inducing hypertension. Renin-angiotensin system (RAS) has been related in PE genesis. It has been reported a novel receptor in the system, the Prorenin/Renin receptor (PRR), with several roles in renal and cardiovascular illnesses. It is not known, however, if PRR changes its expression or is activated during normal or PE-complicated pregnancy on tissues intimately related to hypertension. So, the aim of this work was to describe PRR expression during normal and hypertensive pregnancy in rats.
Methods: We used a subrenal aortic coarctation (SRAC) model in rats. Atria, septum and ventricular heart tissue, aorta and renal tissue samples were homogenized and immunoblotted using anti-PRR and anti-PLZF antibodies. We also measured gene expression by RT-PCR.
Key Findings: Hypertension and proteinuria were observed in SRAC-pregnant rats. In pregnant, non-SRAC rats, PRR showed a higher expression of both, gene and protein compared to non-pregnant rats in heart, aorta and kidney tissues. PE induces a very high expression of PRR in cardiac tissues and, on the contrary, decreases PRR expression in both, aorta and kidney. PLZF, a marker of PRR function, was augmented only in aorta and kidney in non-SRAC pregnant rats. In SRAC-pregnant rats, PLZF increment disappeared.
Significance: These findings indicate that PRR expression changes differently during pregnancy and PE in tissues related to cardiovascular functions and suggest a probable participation of the receptor during normal and preeclamptic pregnancy in the rat.
(Copyright © 2018 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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