| Autor: |
Yarlett N, Rowlands CC, Evans JC, Yarlett NC, Lloyd D |
| Jazyk: |
angličtina |
| Zdroj: |
Molecular and biochemical parasitology [Mol Biochem Parasitol] 1987 Jul; Vol. 24 (3), pp. 255-61. |
| DOI: |
10.1016/0166-6851(87)90157-5 |
| Abstrakt: |
Hydrogenosome-enriched fractions from Trichomonas vaginalis reduce a number of nitroimidazole derivatives to their respective electron spin resonance-detectable nitro-anion radicals. In the presence of of oxygen and 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) a superoxide spin trapped adduct of DMPO was formed; the rate-determining step was the prior formation of the nitro-anion radical. Oxygen-derived radicals were detected with cytosolic fractions from a metronidazole-resistant isolate (CDC-85) when incubated with NADH or NADPH as respiratory substrate. The requirement for superoxide dismutase and catalase to completely abolish formation of these signals suggests contributions from both superoxide and peroxide. No oxygen-derived radicals were observed with cytosolic fractions from a metronidazole-susceptible strain (C1-NIH). |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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