| Autor: |
de Souza MG; Department of Dentistry, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais, Brazil., de Jesus SF; Department of Dentistry, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais, Brazil., Santos EM; Department of Dentistry, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais, Brazil., Gomes ESB; Department of Dentistry, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais, Brazil., de Paulo Santiago Filho A; Hospital Dilson Godinho, Montes Claros, Minas Gerais, Brazil., Santos EMS; Instituto Federal de Educação, Ciência e Tecnologia do Norte de Minas Gerais (IFNMG), Araçuaí, Minas Gerais, Brazil., da Silveira LH; Department of Dentistry, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais, Brazil., Santos SHS; Institute of Agricultural Sciences, Food Engineering College, Universidade Federal de Minas Gerais (UFMG), Montes Claros, Minas Gerais, Brazil., de Paula AMB; Department of Dentistry, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais, Brazil., Farias LC; Department of Dentistry, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais, Brazil., Guimarães ALS; Department of Dentistry, Universidade Estadual de Montes Claros, Montes Claros, Minas Gerais, Brazil. andreluizguimaraes@gmail.com.; Hospital Dilson Godinho, Montes Claros, Minas Gerais, Brazil. andreluizguimaraes@gmail.com.; Hospital Universitário Clemente Faria, Laboratório de Pesquisa em Saúde, Universidade Estadual de Montes Claros, 562 Av. Cula Mangabeira Santo Expedito, Montes Claros, MG, 39401-001, Brazil. andreluizguimaraes@gmail.com. |
| Abstrakt: |
Radiation Therapy (RT) is a treatment option for a large number of neoplasias. However, the effect of RT on the level of hypoxia markers is poorly understood. The present study aimed to investigate the effect of RT on the levels of hypoxic markers in Oral squamous cell carcinoma (OSCC). Evaluation of HIF-1α and miR-210 levels in OSCC was performed. Then a proteomic analysis was performed to identify candidate hypoxic targets of RT. To validate proteomic studies, the effect of RT on HIF-1α, miR-210, PDH-A and LDH-A levels under hypoxia was assessed by qRT-PCR. The impact of RT in hypoxia markers was evaluated in patients to confirm in vitro results. An increase in the HIF-1α levels was observed in OSCC. RT reduced OSCC cell proliferation and migration. Interestingly, hypoxia could revert the effect of radiation on OSCC phenotype. However, proteomics analyses suggested that LDH is one of the critical targets of RT even in hypoxia. Moreover, RT decreased HIF-1α, miR-210, and LDH even in hypoxia. The current study demonstrated that hypoxia could revert the effects of RT in the OSCC context. However, RT reduces the levels HIF-1α, miR-210 and LDH in vivo and in vitro. The consequences of RT in blood should be carefully investigated. |
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