The MAP3K-Coding QUI-GON JINN ( QGJ ) Gene Is Essential to the Formation of Unreduced Embryo Sacs in Paspalum .

Autor:	Mancini M; Instituto de Investigaciones en Ciencias Agrarias de Rosario, CONICET-UNR, Facultad de Ciencias Agrarias, Universidad Nacional de Rosario, Zavalla, Argentina., Permingeat H; Instituto de Investigaciones en Ciencias Agrarias de Rosario, CONICET-UNR, Facultad de Ciencias Agrarias, Universidad Nacional de Rosario, Zavalla, Argentina., Colono C; Instituto de Investigaciones en Ciencias Agrarias de Rosario, CONICET-UNR, Facultad de Ciencias Agrarias, Universidad Nacional de Rosario, Zavalla, Argentina., Siena L; Instituto de Investigaciones en Ciencias Agrarias de Rosario, CONICET-UNR, Facultad de Ciencias Agrarias, Universidad Nacional de Rosario, Zavalla, Argentina., Pupilli F; Istituto di Bioscienze e BioRisorse, Consiglio Nazionale delle Ricerche, Perugia, Italy., Azzaro C; Instituto de Investigaciones en Ciencias Agrarias de Rosario, CONICET-UNR, Facultad de Ciencias Agrarias, Universidad Nacional de Rosario, Zavalla, Argentina., de Alencar Dusi DM; Parque Estação Biológica, Embrapa Recursos Genéticos e Biotecnologia, Brasília, Brazil., de Campos Carneiro VT; Parque Estação Biológica, Embrapa Recursos Genéticos e Biotecnologia, Brasília, Brazil., Podio M; Instituto de Investigaciones en Ciencias Agrarias de Rosario, CONICET-UNR, Facultad de Ciencias Agrarias, Universidad Nacional de Rosario, Zavalla, Argentina., Seijo JG; Instituto de Botánica del Nordeste, CONICET-UNNE, Corrientes, Argentina.; Facultad de Ciencias Exactas y Naturales y Agrimensura, Universidad Nacional del Nordeste, Corrientes, Argentina., González AM; Instituto de Botánica del Nordeste, CONICET-UNNE, Corrientes, Argentina.; Facultad de Ciencias Agrarias, Universidad Nacional del Nordeste, Corrientes, Argentina., Felitti SA; Instituto de Investigaciones en Ciencias Agrarias de Rosario, CONICET-UNR, Facultad de Ciencias Agrarias, Universidad Nacional de Rosario, Zavalla, Argentina., Ortiz JPA; Instituto de Investigaciones en Ciencias Agrarias de Rosario, CONICET-UNR, Facultad de Ciencias Agrarias, Universidad Nacional de Rosario, Zavalla, Argentina., Leblanc O; DIADE, Univ Montpellier, IRD, Montpellier, France., Pessino SC; Instituto de Investigaciones en Ciencias Agrarias de Rosario, CONICET-UNR, Facultad de Ciencias Agrarias, Universidad Nacional de Rosario, Zavalla, Argentina.
Jazyk:	angličtina
Zdroj:	Frontiers in plant science [Front Plant Sci] 2018 Oct 24; Vol. 9, pp. 1547. Date of Electronic Publication: 2018 Oct 24 (Print Publication: 2018).
DOI:	10.3389/fpls.2018.01547
Abstrakt:	Apomixis is a clonal mode of reproduction via seeds, which results from the failure of meiosis and fertilization in the sexual female reproductive pathway. In previous transcriptomic surveys, we identified a mitogen-activated protein kinase kinase kinase (N46) displaying differential representation in florets of sexual and apomictic Paspalum notatum genotypes. Here, we retrieved and characterized the N46 full cDNA sequence from sexual and apomictic floral transcriptomes. Phylogenetic analyses showed that N46 was a member of the YODA family, which was re-named QUI-GON JINN ( QGJ ). Differential expression in florets of sexual and apomictic plants was confirmed by qPCR. In situ hybridization experiments revealed expression in the nucellus of aposporous plants' ovules, which was absent in sexual plants. RNAi inhibition of QGJ expression in two apomictic genotypes resulted in significantly reduced rates of aposporous embryo sac formation, with respect to the level detected in wild type aposporous plants and transformation controls. The QGJ locus segregated independently of apospory. However, a probe derived from a related long non-coding RNA sequence ( PN_LNC_QGJ ) revealed RFLP bands cosegregating with the Paspalum apospory-controlling region (ACR). PN_LNC_QGJ is expressed in florets of apomictic plants only. Our results indicate that the activity of QGJ in the nucellus of apomictic plants is necessary to form non-reduced embryo sacs and that a long non-coding sequence with regulatory potential is similar to sequences located within the ACR.
Databáze:	MEDLINE
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