The effect of hyperglycemia on neurovascular coupling and cerebrovascular patterning in zebrafish.

Autor: Chhabria K; Neuroimaging in Cardiovascular Disease (NICAD) Network, University of Sheffield, Sheffield, UK.; Department of Infection, Immunity and Cardiovascular Disease, University of Sheffield Medical School, Sheffield, UK.; The Bateson Centre, University of Sheffield, Sheffield, UK., Plant K; Department of Infection, Immunity and Cardiovascular Disease, University of Sheffield Medical School, Sheffield, UK.; The Bateson Centre, University of Sheffield, Sheffield, UK., Bandmann O; The Bateson Centre, University of Sheffield, Sheffield, UK.; Department of Neuroscience, University of Sheffield Medical School, Sheffield, UK., Wilkinson RN; Department of Infection, Immunity and Cardiovascular Disease, University of Sheffield Medical School, Sheffield, UK.; The Bateson Centre, University of Sheffield, Sheffield, UK., Martin C; Neuroimaging in Cardiovascular Disease (NICAD) Network, University of Sheffield, Sheffield, UK.; Department of Psychology, University of Sheffield, Sheffield, UK., Kugler E; Department of Infection, Immunity and Cardiovascular Disease, University of Sheffield Medical School, Sheffield, UK.; The Bateson Centre, University of Sheffield, Sheffield, UK., Armitage PA; Neuroimaging in Cardiovascular Disease (NICAD) Network, University of Sheffield, Sheffield, UK.; Department of Infection, Immunity and Cardiovascular Disease, University of Sheffield Medical School, Sheffield, UK., Santoscoy PL; The Bateson Centre, University of Sheffield, Sheffield, UK.; Department of Biomedical Science, University of Sheffield, Sheffield, UK., Cunliffe VT; The Bateson Centre, University of Sheffield, Sheffield, UK.; Department of Biomedical Science, University of Sheffield, Sheffield, UK., Huisken J; Morgridge Institute for Research, Madison, WI, USA., McGown A; The Bateson Centre, University of Sheffield, Sheffield, UK.; Department of Neuroscience, University of Sheffield Medical School, Sheffield, UK., Ramesh T; The Bateson Centre, University of Sheffield, Sheffield, UK.; Department of Neuroscience, University of Sheffield Medical School, Sheffield, UK., Chico TJ; Neuroimaging in Cardiovascular Disease (NICAD) Network, University of Sheffield, Sheffield, UK.; Department of Infection, Immunity and Cardiovascular Disease, University of Sheffield Medical School, Sheffield, UK.; The Bateson Centre, University of Sheffield, Sheffield, UK., Howarth C; Neuroimaging in Cardiovascular Disease (NICAD) Network, University of Sheffield, Sheffield, UK.; Department of Psychology, University of Sheffield, Sheffield, UK.
Jazyk: angličtina
Zdroj: Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism [J Cereb Blood Flow Metab] 2020 Feb; Vol. 40 (2), pp. 298-313. Date of Electronic Publication: 2018 Nov 06.
DOI: 10.1177/0271678X18810615
Abstrakt: Neurovascular coupling (through which local cerebral blood flow changes in response to neural activation are mediated) is impaired in many diseases including diabetes. Current preclinical rodent models of neurovascular coupling rely on invasive surgery and instrumentation, but transgenic zebrafish coupled with advances in imaging techniques allow non-invasive quantification of cerebrovascular anatomy, neural activation, and cerebral vessel haemodynamics. We therefore established a novel non-invasive, non-anaesthetised zebrafish larval model of neurovascular coupling, in which visual stimulus evokes neuronal activation in the optic tectum that is associated with a specific increase in red blood cell speed in tectal blood vessels. We applied this model to the examination of the effect of glucose exposure on cerebrovascular patterning and neurovascular coupling. We found that chronic exposure of zebrafish to glucose impaired tectal blood vessel patterning and neurovascular coupling. The nitric oxide donor sodium nitroprusside rescued all these adverse effects of glucose exposure on cerebrovascular patterning and function. Our results establish the first non-mammalian model of neurovascular coupling, offering the potential to perform more rapid genetic modifications and high-throughput screening than is currently possible using rodents. Furthermore, using this zebrafish model, we reveal a potential strategy to ameliorate the effects of hyperglycemia on cerebrovascular function.
Databáze: MEDLINE