Outbreak of viral haemorrhagic septicaemia (VHS) in lumpfish (Cyclopterus lumpus) in Iceland caused by VHS virus genotype IV.

Autor: Guðmundsdóttir S; Fish Disease Laboratory, Institute for Experimental Pathology, University of Iceland, Reykjavík, Iceland., Vendramin N; European Union Reference Laboratory for Fish Diseases, National Institute for Aquatic Resources, Technical University of Denmark, Copenhagen, Denmark., Cuenca A; European Union Reference Laboratory for Fish Diseases, National Institute for Aquatic Resources, Technical University of Denmark, Copenhagen, Denmark., Sigurðardóttir H; Fish Disease Laboratory, Institute for Experimental Pathology, University of Iceland, Reykjavík, Iceland., Kristmundsson A; Fish Disease Laboratory, Institute for Experimental Pathology, University of Iceland, Reykjavík, Iceland., Iburg TM; European Union Reference Laboratory for Fish Diseases, National Institute for Aquatic Resources, Technical University of Denmark, Copenhagen, Denmark., Olesen NJ; European Union Reference Laboratory for Fish Diseases, National Institute for Aquatic Resources, Technical University of Denmark, Copenhagen, Denmark.
Jazyk: angličtina
Zdroj: Journal of fish diseases [J Fish Dis] 2019 Jan; Vol. 42 (1), pp. 47-62. Date of Electronic Publication: 2018 Nov 05.
DOI: 10.1111/jfd.12910
Abstrakt: A novel viral haemorrhagic septicaemia virus (VHSV) of genotype IV was isolated from wild lumpfish (Cyclopterus lumpus), brought to a land-based farm in Iceland, to serve as broodfish. Two groups of lumpfish juveniles, kept in tanks in the same facility, got infected. The virus isolated was identified as VHSV by ELISA and real-time RT-PCR. Phylogenetic analysis, based on the glycoprotein (G) gene sequences, may indicate a novel subgroup of VHSV genotype IV. In controlled laboratory exposure studies with this new isolate, there was 3% survival in the I.P. injection challenged group while there was 90% survival in the immersion group. VHSV was not re-isolated from fish challenged by immersion. In a cohabitation trial, lumpfish infected I.P. (shedders) were placed in tanks with naïve lumpfish as well as naïve Atlantic salmon (Salmo salar L.). 10% of the lumpfish shedders and 43%-50% of the cohabiting lumpfish survived after 4 weeks. 80%-92% of the Atlantic salmon survived, but no viral RNA was detected by real-time RT-PCR nor VHSV was isolated from Atlantic salmon. This is the first isolation of a notifiable virus in Iceland and the first report of VHSV of genotype IV in European waters.
(© 2018 The Authors. Journal of Fish Diseases Published by John Wiley & Sons Ltd.)
Databáze: MEDLINE
Externí odkaz: