Peptidylarginine deiminase and deiminated proteins are detected throughout early halibut ontogeny - Complement components C3 and C4 are post-translationally deiminated in halibut (Hippoglossus hippoglossus L.).

Autor: Magnadóttir B; Institute for Experimental Pathology, University of Iceland, Keldur v. Vesturlandsveg, 112 Reykjavik, Iceland. Electronic address: bergmagn@hi.is., Bragason BT; Institute for Experimental Pathology, University of Iceland, Keldur v. Vesturlandsveg, 112 Reykjavik, Iceland. Electronic address: birkirbr@hi.is., Bricknell IR; Aquaculture Research Institute School of Marine Sciences, University of Maine, Orono, ME, USA. Electronic address: ian.bricknell@maine.edu., Bowden T; Aquaculture Research Institute School of Food & Agriculture, University of Maine, University of Maine, Orono, ME, USA. Electronic address: timothy.bowden@maine.edu., Nicholas AP; Department of Neurology, University of Alabama at Birmingham, Birmingham, AL, USA. Electronic address: anicholas@uabmc.edu., Hristova M; Perinatal Brain Protection and Repair Group, EGA Institute for Women's Health, University College London, London, WC1E 6HX, UK. Electronic address: m.hristova@ucl.ac.uk., Guðmundsdóttir S; Institute for Experimental Pathology, University of Iceland, Keldur v. Vesturlandsveg, 112 Reykjavik, Iceland. Electronic address: siggag@hi.is., Dodds AW; MRC Immunochemistry Unit, Department of Biochemistry, University of Oxford, Oxford, UK. Electronic address: awdodds@gmail.com., Lange S; Tissue Architecture and Regeneration Research Group, School of Life Sciences, University of Westminster, London, W1W 6UW, UK. Electronic address: S.Lange@westminster.ac.uk.
Jazyk: angličtina
Zdroj: Developmental and comparative immunology [Dev Comp Immunol] 2019 Mar; Vol. 92, pp. 1-19. Date of Electronic Publication: 2018 Nov 03.
DOI: 10.1016/j.dci.2018.10.016
Abstrakt: Post-translational protein deimination is mediated by peptidylarginine deiminases (PADs), which are calcium dependent enzymes conserved throughout phylogeny with physiological and pathophysiological roles. Protein deimination occurs via the conversion of protein arginine into citrulline, leading to structural and functional changes in target proteins. In a continuous series of early halibut development from 37 to 1050° d, PAD, total deiminated proteins and deiminated histone H3 showed variation in temporal and spatial detection in various organs including yolksac, muscle, skin, liver, brain, eye, spinal cord, chondrocytes, heart, intestines, kidney and pancreas throughout early ontogeny. For the first time in any species, deimination of complement components C3 and C4 is shown in halibut serum, indicating a novel mechanism of complement regulation in immune responses and homeostasis. Proteomic analysis of deiminated target proteins in halibut serum further identified complement components C5, C7, C8 C9 and C1 inhibitor, as well as various other immunogenic, metabolic, cytoskeletal and nuclear proteins. Post-translational deimination may facilitate protein moonlighting, an evolutionary conserved phenomenon, allowing one polypeptide chain to carry out various functions to meet functional requirements for diverse roles in immune defences and tissue remodelling.
(Copyright © 2018 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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