Non-coding RNA Expression, Function, and Variation during Drosophila Embryogenesis.

Autor:	Schor IE; Genome Biology Unit, European Molecular Biology Laboratory (EMBL), 69117 Heidelberg, Germany; Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE-CONICET), Departamento de Fisiología, Biología Molecular y Celular, FCEyN, Universidad de Buenos Aires, Buenos Aires, Argentina., Bussotti G; European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Hinxton CB10 1SD, UK., Maleš M; Genome Biology Unit, European Molecular Biology Laboratory (EMBL), 69117 Heidelberg, Germany., Forneris M; Genome Biology Unit, European Molecular Biology Laboratory (EMBL), 69117 Heidelberg, Germany., Viales RR; Genome Biology Unit, European Molecular Biology Laboratory (EMBL), 69117 Heidelberg, Germany., Enright AJ; European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Hinxton CB10 1SD, UK., Furlong EEM; Genome Biology Unit, European Molecular Biology Laboratory (EMBL), 69117 Heidelberg, Germany. Electronic address: furlong@embl.de.
Jazyk:	angličtina
Zdroj:	Current biology : CB [Curr Biol] 2018 Nov 19; Vol. 28 (22), pp. 3547-3561.e9. Date of Electronic Publication: 2018 Nov 01.
DOI:	10.1016/j.cub.2018.09.026
Abstrakt:	Long non-coding RNAs (lncRNAs) can often function in the regulation of gene expression during development; however, their generality as essential regulators in developmental processes and organismal phenotypes remains unclear. Here, we performed a tailored investigation of lncRNA expression and function during Drosophila embryogenesis, interrogating multiple stages, tissue specificity, nuclear localization, and genetic backgrounds. Our results almost double the number of annotated lncRNAs expressed at these embryonic stages. lncRNA levels are generally positively correlated with those of their neighboring genes, with little evidence of transcriptional interference. Using fluorescent in situ hybridization, we report the spatiotemporal expression of 15 new lncRNAs, revealing very dynamic tissue-specific patterns. Despite this, deletion of selected lncRNA genes had no obvious developmental defects or effects on viability under standard and stressed conditions. However, two lncRNA deletions resulted in modest expression changes of a small number of genes, suggesting that they fine-tune expression of non-essential genes. Several lncRNAs have strain-specific expression, indicating that they are not fixed within the population. This intra-species variation across genetic backgrounds may thereby be a useful tool to distinguish rapidly evolving lncRNAs with as yet non-essential roles. (Copyright © 2018 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
Databáze:	MEDLINE
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