Characterization of Candida species isolated from the hands of the healthcare workers in the neonatal intensive care unit.

Autor:	de Paula Menezes R; HealthTechnical School- ESTES, Federal University of Uberlandia (UFU), Uberlandia, MG, Brazil.; Health Sciences Postgraduate Program, Faculty of Medicine- FAMED, UFU, Uberlandia, MG, Brazil., Silva FF; Health Sciences Postgraduate Program, Faculty of Medicine- FAMED, UFU, Uberlandia, MG, Brazil., Melo SGO; Undergraduation in Nursing, FAMED, UFU, Uberlandia, MG., Alves PGV; Health Sciences Postgraduate Program, Faculty of Medicine- FAMED, UFU, Uberlandia, MG, Brazil., Brito MO; Health Sciences Postgraduate Program, Faculty of Medicine- FAMED, UFU, Uberlandia, MG, Brazil., de Souza Bessa MA; Undergraduation in Biology, Institute of Biology (INBIO), UFU, Uberlandia, MG, Brazil., Amante Penatti MP; HealthTechnical School- ESTES, Federal University of Uberlandia (UFU), Uberlandia, MG, Brazil., Pedroso RS; HealthTechnical School- ESTES, Federal University of Uberlandia (UFU), Uberlandia, MG, Brazil.; Health Sciences Postgraduate Program, Faculty of Medicine- FAMED, UFU, Uberlandia, MG, Brazil., Abdallah VOS; Health Sciences Postgraduate Program, Faculty of Medicine- FAMED, UFU, Uberlandia, MG, Brazil., Röder DVDB; Health Sciences Postgraduate Program, Faculty of Medicine- FAMED, UFU, Uberlandia, MG, Brazil.; Institute of Biomedical Sciences, ICBIM, UFU, Uberlandia, MG, Brazil.
Jazyk:	angličtina
Zdroj:	Medical mycology [Med Mycol] 2019 Jul 01; Vol. 57 (5), pp. 588-594.
DOI:	10.1093/mmy/myy101
Abstrakt:	Colonization of health professional hands by potentially pathogenic microorganisms capable to expressing virulence factors, such as Candida spp., is worrisome because of the high contact between patients and professionals. The study aims to evaluate, in vitro, the following virulence factors: hemolytic activity, DNAse expression, biofilm formation, and susceptibility for antifungal agents of Candida species isolated from health professionals hands of a neonatal intensive care unit (NICU). The study includes 50 isolates of Candida spp.: 19 C. parapsilosis sensu stricto, three C. metapsilosis, one C. orthopsilosis, seven C. albicans, six C. famata, five C. lusitaniae, three C. krusei, two C. kefyr, two C. tropicalis, one C. glabrata, and one C. guilliermondii. The hemolytic activity and DNAse were investigated using blood agar and DNAse agar, respectively. Biofilm production was evaluated through XTT sodium salt reduction ability, and the susceptibility of the isolates to antifungals through the microdilution methodology. Forty-nine isolates presented at least one of the three virulence factors investigated. C. albicans showed more intense hemolytic activity. DNAse production was statistically significant between the C. parapsilosis complex and C. albicans, as well as between the C. parapsilosis complex and C. krusei, even as between C. famata and C. albicans, and between C. famata and C. krusei. Forty-three isolates produced biofilm. Seventy-eight percent of the isolates were sensitive to the three antifungals tested. This study demonstrated that Candida isolated from healthcare professionals' hands has virulence. (© The Author(s) 2018. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology.)
Databáze:	MEDLINE
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