Search for proteomic biomarkers related to bovine pre-slaughter stress using liquid isoelectric focusing (OFFGEL) and mass spectrometry.

Autor: Fuente-Garcia C; Instituto de Agroquímica y Tecnología de Alimentos (CSIC), 46980 Paterna, Valencia, Spain; Lactiker Research Group, Department of Pharmacy and Food Sciences, University of the Basque Country (UPV/EHU), 01006 Vitoria-Gasteiz, Spain., Aldai N; Lactiker Research Group, Department of Pharmacy and Food Sciences, University of the Basque Country (UPV/EHU), 01006 Vitoria-Gasteiz, Spain., Sentandreu E; Instituto de Agroquímica y Tecnología de Alimentos (CSIC), 46980 Paterna, Valencia, Spain., Oliván M; Servicio Regional de Investigación y Desarrollo Alimentario (SERIDA), 33300 Villaviciosa, Asturias, Spain., García-Torres S; Centro de Investigaciones Científicas y Tecnológicas de Extremadura (CICYTEX), 06187 Guadajira, Badajoz, Spain., Franco D; Centro Tecnolóxico da Carne (CETECA), 32900 San Cibrao das Viñas, Ourense, Spain., Zapata C; Department of Zoology, Genetics and Physical Anthropology, University of Santiago de Compostela (USC), 15782 Santiago de Compostela, La Coruña, Spain., Sentandreu MA; Instituto de Agroquímica y Tecnología de Alimentos (CSIC), 46980 Paterna, Valencia, Spain. Electronic address: ciesen@iata.csic.es.
Jazyk: angličtina
Zdroj: Journal of proteomics [J Proteomics] 2019 Apr 30; Vol. 198, pp. 59-65. Date of Electronic Publication: 2018 Oct 29.
DOI: 10.1016/j.jprot.2018.10.013
Abstrakt: Proteome changes derived from animals that have suffered pre-slaughter stress are a fact. In this study, Proteomic analysis was carried out on 20 bovine loin samples from Asturiana de los Valles and crossbreds cattle previously classified as normal and DFD meat at 24 h post-mortem using pH measurements. Sarcoplasmic sub-proteome of Longissimus thoracis at 24 h post-mortem was fractionated by the use of liquid isoelectric focusing (OFFGEL) in the pH range 3-10, followed by SDS-PAGE analysis of each retrieved fraction. The protein fractionation profile showed high reproducibility along the different sample groups. Five protein bands showed significant differences (p < 0.05) between the two groups, allowing discrimination between them. Proteins present in these bands, which were identified by LC-MS, were actin, phosphoglucomutase-1, alpha-crystallin B, heat shock protein beta-6 and heat shock protein beta-1. SIGNIFICANCE: The significance of this study relies on the optimization of OFFGEL fractionation as a promising technology to search for reliable biomarkers of pre-slaughter stress. This method separates proteins along different liquid fractions according to their isoelectric point; the obtained fractions can be further characterized by SDS-PAGE or directly identified by LC-MS. This achievement stands out as an alternative to the use of 2-DE electrophoresis in protein separation and analysis.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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