In vitro tracking of phospholipase A 2 from snake venom conjugated with magic-sized quantum dots.

Autor: Dias EHV; Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Uberlândia, MG, Brazil., Pereira DFC; Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Uberlândia, MG, Brazil., de Sousa BB; Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Uberlândia, MG, Brazil; Instituto de Química, Universidade Estadual Paulista, Araraquara, SP, Brazil., Matias MS; Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Uberlândia, MG, Brazil., de Queiroz MR; Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Uberlândia, MG, Brazil; Instituto Nacional de Ciência e Tecnologia em Nano-Biofarmacêutica (N-Biofar), Belo Horizonte, MG, Brazil., Santiago FM; Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia, MG, Brazil., Silva ACA; Instituto de Física, Universidade Federal de Uberlândia, Uberlândia, MG, Brazil., Dantas NO; Instituto de Física, Universidade Federal de Uberlândia, Uberlândia, MG, Brazil., Santos-Filho NA; Instituto de Química, Universidade Estadual Paulista, Araraquara, SP, Brazil., de Oliveira F; Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia, MG, Brazil; Instituto Nacional de Ciência e Tecnologia em Nano-Biofarmacêutica (N-Biofar), Belo Horizonte, MG, Brazil. Electronic address: fabio@ufu.br.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2019 Feb 01; Vol. 122, pp. 461-468. Date of Electronic Publication: 2018 Oct 29.
DOI: 10.1016/j.ijbiomac.2018.10.185
Abstrakt: Phospholipases A 2 represent a family of enzymes with important application in medicine. However, direct tracking is difficult due to the absence of a stable, effective and specific marker for these enzymes. Magic-sized quantum dots (MSQDs) are inorganic semiconducting nanocrystals with unique physical properties. They have the ability to conjugate to proteins, making them excellent markers for biological systems. In this work, we labelled phospholipase A 2 from Bothrops alternatus snake venom with Cadmium selenide (CdSe)/cadmium sulphate (CdS) MSQDs-a biocompatible and luminescent probe-. Bioconjugation was confirmed using infrared spectra and fluorescence microscopy, which demonstrated that the CdSe/CdS MSQDs interact with phospholipase A 2 without interfering with its activity. This probe may be an important tool for the elucidation of many biological mechanisms, because it allows the pathway of phospholipase A 2 to be tracked from its entry through the plasma membrane until its incorporation into the nucleus of myoblasts.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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