| Autor: |
Youssef MAM; a Children Hospital, Faculty of Medicine , Assiut University , Egypt., Zahran AM; b Clinical Pathology Department, South Egypt Cancer Institute , Assiut University , Assiut , Egypt., Hussien AM; a Children Hospital, Faculty of Medicine , Assiut University , Egypt., Elsayh KI; a Children Hospital, Faculty of Medicine , Assiut University , Egypt., Askar EA; a Children Hospital, Faculty of Medicine , Assiut University , Egypt., Farghaly HS; a Children Hospital, Faculty of Medicine , Assiut University , Egypt. |
| Jazyk: |
angličtina |
| Zdroj: |
Paediatrics and international child health [Paediatr Int Child Health] 2019 May; Vol. 39 (2), pp. 111-118. Date of Electronic Publication: 2018 Oct 30. |
| DOI: |
10.1080/20469047.2018.1528755 |
| Abstrakt: |
Background : Vitamin D has regulatory effects on different cells of the immune system and low levels are associated with several immune-mediated diseases. Aim : To investigate the association between neonatal 25-hydroxy vitamin D (25-OHD) level and the expression of lymphocyte activation markers (HLA-DR, CD69, CD25, CD45RA) on T-lymphocyte subpopulations and its impact in neonatal infection. Methods : 25-OHD level was measured in the cord blood of 56 neonates and their mothers using an enzyme immune-assay method. Based on the 25-OHD level, infants were categorised into four groups: severe deficiency ( n = 7), moderate deficiency ( n = 21), mild deficiency ( n = 15) and normal 25-OHD level ( n = 13). Mothers were classified into deficient ( n = 18), insufficient ( n = 21) and normal levels ( n = 17). T-lymphocyte subpopulations and lymphocyte activation markers were investigated using flow cytometry. Results : There was a positive correlation between maternal and cord blood 25-OHD levels ( r = 0.503, p = 0.001). The group with severe 25-OHD deficiency had the significantly lowest level of total lymphocytes, CD3+ T lymphocytes, CD4+ T-helper and CD8+ T-cytotoxic lymphocytes and CD4+CD45RA+ naïve T-cells compared with the other groups. The frequencies of CD8+CD25+, CD4+CD25+ and CD4+HLA-DR+ activated T-lymphocytes were significantly lower in the severe, moderate and mild deficiency groups than in the normal group. Seven of 43 (16.27%) infants with 25-OHD deficiency were admitted with sepsis to the neonatal intensive care unit and there were no cases of sepsis in the normal 25-OHD group. Conclusion : Vitamin D deficiency is associated with a reduction of lymphocyte subsets and altered T-lymphocyte activation which are considered to be risk factors for neonatal infection. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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