Overexpressing ovotransferrin and avian β-defensin-3 improves antimicrobial capacity of chickens and poultry products.

Autor: Cooper CA; CSIRO Health and Biosecurity, Geelong, VIC, 3219, Australia. Caitlin.Cooper@csiro.au., Tizard ML; CSIRO Health and Biosecurity, Geelong, VIC, 3219, Australia., Stanborough T; CSIRO Agriculture and Food, Werribee, VIC, 3030, Australia., Moore SC; CSIRO Agriculture and Food, Werribee, VIC, 3030, Australia., Chandry PS; CSIRO Agriculture and Food, Werribee, VIC, 3030, Australia., Jenkins KA; CSIRO Health and Biosecurity, Geelong, VIC, 3219, Australia., Wise TG; CSIRO Health and Biosecurity, Geelong, VIC, 3219, Australia., O'Neil TE; CSIRO Health and Biosecurity, Geelong, VIC, 3219, Australia., Layton DS; CSIRO Health and Biosecurity, Geelong, VIC, 3219, Australia., Morris KR; CSIRO Health and Biosecurity, Geelong, VIC, 3219, Australia., Moore RJ; School of Science, RMIT University, Bundoora, VIC, 3083, Australia., Fegan N; CSIRO Agriculture and Food, Werribee, VIC, 3030, Australia., Doran TJ; CSIRO Health and Biosecurity, Geelong, VIC, 3219, Australia.
Jazyk: angličtina
Zdroj: Transgenic research [Transgenic Res] 2019 Feb; Vol. 28 (1), pp. 51-76. Date of Electronic Publication: 2018 Oct 29.
DOI: 10.1007/s11248-018-0101-2
Abstrakt: Zoonotic and foodborne diseases pose a significant burden, decreasing both human and animal health. Modifying chickens to overexpress antimicrobials has the potential to decrease bacterial growth on poultry products and boost chicken innate immunity. Chickens overexpressing either ovotransferrin or avian β-defensin-3 (AvβD3) were generated using Tol-2 transposons. Transgene expression at the RNA and protein level was seen in egg white, breast muscle, and serum. There were significant differences in the immune cell populations in the blood, bursa, and spleen associated with transgene expression including an increased proportion of CD8+ cells in the blood of ovotransferrin and AvβD3 transgenic birds. Expression of the antimicrobials inhibited the in vitro growth of human and chicken bacterial pathogens and spoilage bacteria. For example, transgene expression significantly reduced growth of aerobic and coliform bacteria in breast muscle and decreased the growth of Salmonella enterica in egg white. Overall these results indicate that overexpression of antimicrobials in the chicken can impact the immune system and increase the antimicrobial capacity of poultry products.
Databáze: MEDLINE
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