Modified cantilever arrays improve sensitivity and reproducibility of nanomechanical sensing in living cells.

Autor: Patil SB; London Centre for Nanotechnology, 17-19 Gordon Street, WC1H 0AH and Division of Medicine, 5 University Street, WC1E 6JF, University College London, London, UK., Al-Jehani RM; UCL Institute for Liver and Digestive Health, Royal Free Hospital and NHS Foundation Trust, London, NW3 2QG, UK., Etayash H; Department of Chemical and Materials Engineering, University of Alberta, Edmonton, AB, T6G 2V4, Canada.; Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, AB, T6G 2E1, Canada., Turbe V; London Centre for Nanotechnology, 17-19 Gordon Street, WC1H 0AH and Division of Medicine, 5 University Street, WC1E 6JF, University College London, London, UK., Jiang K; Department of Chemical and Materials Engineering, University of Alberta, Edmonton, AB, T6G 2V4, Canada., Bailey J; London Centre for Nanotechnology, 17-19 Gordon Street, WC1H 0AH and Division of Medicine, 5 University Street, WC1E 6JF, University College London, London, UK., Al-Akkad W; UCL Institute for Liver and Digestive Health, Royal Free Hospital and NHS Foundation Trust, London, NW3 2QG, UK., Soudy R; Department of Medicine, Neuroscience and Mental Health Institute, University of Alberta, 530 Heritage Medical Research Centre, Edmonton, AB, T6G 2S2, Canada., Kaur K; Chapman University School of Pharmacy (CUSP), Harry and Diane Rinker Health Science Campus, Chapman University, Irvine, CA, 92618-1908, USA., McKendry RA; London Centre for Nanotechnology, 17-19 Gordon Street, WC1H 0AH and Division of Medicine, 5 University Street, WC1E 6JF, University College London, London, UK., Thundat T; Department of Chemical and Materials Engineering, University of Alberta, Edmonton, AB, T6G 2V4, Canada., Ndieyira JW; London Centre for Nanotechnology, 17-19 Gordon Street, WC1H 0AH and Division of Medicine, 5 University Street, WC1E 6JF, University College London, London, UK. j.ndieyira@ucl.ac.uk.; UCL Institute for Liver and Digestive Health, Royal Free Hospital and NHS Foundation Trust, London, NW3 2QG, UK. j.ndieyira@ucl.ac.uk.; Department of Chemistry, Jomo Kenyatta University of Agriculture and Technology, PO Box 62000, Nairobi, Kenya. j.ndieyira@ucl.ac.uk.
Jazyk: angličtina
Zdroj: Communications biology [Commun Biol] 2018 Oct 24; Vol. 1, pp. 175. Date of Electronic Publication: 2018 Oct 24 (Print Publication: 2018).
DOI: 10.1038/s42003-018-0179-3
Abstrakt: Mechanical signaling involved in molecular interactions lies at the heart of materials science and biological systems, but the mechanisms involved are poorly understood. Here we use nanomechanical sensors and intact human cells to provide unique insights into the signaling pathways of connectivity networks, which deliver the ability to probe cells to produce biologically relevant, quantifiable and reproducible signals. We quantify the mechanical signals from malignant cancer cells, with 10 cells per ml in 1000-fold excess of non-neoplastic human epithelial cells. Moreover, we demonstrate that a direct link between cells and molecules creates a continuous connectivity which acts like a percolating network to propagate mechanical forces over both short and long length-scales. The findings provide mechanistic insights into how cancer cells interact with one another and with their microenvironments, enabling them to invade the surrounding tissues. Further, with this system it is possible to understand how cancer clusters are able to co-ordinate their migration through narrow blood capillaries.
Competing Interests: The authors declare no competing interests.
Databáze: MEDLINE
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